PUBLICATION
Transcriptomic characterization of zebrafish larvae in response to mercury exposure
- Authors
- Lu, X., Xiang, Y., Yang, G., Zhang, L., Wang, H., Zhong, S.
- ID
- ZDB-PUB-161215-26
- Date
- 2017
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 192: 40-49 (Journal)
- Registered Authors
- Wang, Hui
- Keywords
- Abcb5, Gene expression, Mercury, RNA-seq, Zebrafish
- MeSH Terms
-
- Transfection
- Animals
- Gene Regulatory Networks
- Databases, Genetic
- Biological Transport
- Gene Expression Regulation, Developmental/drug effects*
- Transcription, Genetic/drug effects*
- Larva/drug effects*
- Larva/genetics
- Gene Expression Profiling/methods
- Mercuric Chloride/metabolism
- Mercuric Chloride/toxicity*
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- LLC-PK1 Cells
- Computational Biology
- Swine
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Time Factors
- ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics
- ATP Binding Cassette Transporter, Subfamily B, Member 1/metabolism
- High-Throughput Nucleotide Sequencing
- PubMed
- 27939723 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Lu, X., Xiang, Y., Yang, G., Zhang, L., Wang, H., Zhong, S. (2017) Transcriptomic characterization of zebrafish larvae in response to mercury exposure. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 192:40-49.
Abstract
Mercury is a widespread toxicant in aquatic environment that can cause deleterious effects on fish. Although a number of mercury-regulated genes have been investigated in adult fish, the transcriptional responses of fish larvae to acute mercury exposure are not well understood. In this study, RNA sequencing was used to examine the transcriptional changes in developing zebrafish larvae under a low concentration of mercuric chloride exposure from 24 to 120hpf. Our initial results showed that a total of 142.59 million raw reads were obtained from sequencing libraries and about 86% of the processed reads were mapped to the reference genome of zebrafish. Differential expression analysis identified 391 up- and 87 down-regulated genes. Gene ontology enrichment analysis revealed that most of the differential expressed genes are closely related to the regulation of cellular process, metabolic process, multicellular organismal process, biological regulation, pigmentation, and response to stimulus. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis demonstrated that antigen processing and presentation was the most significantly enriched pathway. Moreover, we characterized a novel and sensitive mercury-induced ABCB (ATP- binding cassette B subfamily) transporter gene - abcb5. This gene is localized on zebrafish chromosome 16 and contains a 4014bp open-reading frame. The deduced polypeptide is composed of 1337 amino acids and possesses most of functional domains and critical residues defined in human and mouse ABCB5/Abcb5. Functional analysis in vitro demonstrated that overexpression of zebrafish abcb5 gene can significantly decrease the cytotoxicity of mercury in LLC-PK1 cells, implying it is a potential efflux transporter of mercury. Thus, these findings provide useful insights to help further understand the transcriptional response and detoxification ability of zebrafish larvae following acute exposure to mercury.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping