PUBLICATION
Basagran® induces developmental malformations and changes the bacterial community of zebrafish embryos.
- Authors
- Oliveira, J.M., Galhano, V., Henriques, I., Soares, A.M., Loureiro, S.
- ID
- ZDB-PUB-161204-5
- Date
- 2017
- Source
- Environmental pollution (Barking, Essex : 1987) 221: 52-63 (Journal)
- Registered Authors
- Loureiro, Susana, Soares, Amadeu
- Keywords
- Bacterial communities, Basagran(®), DGGE, Embryotoxicity, Zebrafish
- MeSH Terms
-
- Animals
- Bacteria/drug effects*
- Benzothiadiazines/toxicity*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/microbiology*
- Embryonic Development/drug effects
- Herbicides/toxicity*
- Larva
- Swimming
- Zebrafish/embryology*
- PubMed
- 27913070 Full text @ Environ. Pollut.
Citation
Oliveira, J.M., Galhano, V., Henriques, I., Soares, A.M., Loureiro, S. (2017) Basagran® induces developmental malformations and changes the bacterial community of zebrafish embryos.. Environmental pollution (Barking, Essex : 1987). 221:52-63.
Abstract
This study aimed to assess the effects of Basagran® on zebrafish (Danio rerio) embryos. The embryos were exposed to Basagran® at concentrations ranging from 120.0 to 480.6 mg/L, and the effects on embryo development (up to 96 h) and bacterial communities of 96 h-larvae were assessed. The embryo development response was time-dependent and concentration-dependent (106.35 < EC50 < 421.58 mg/L). The sensitivity of embryo-related endpoints decreased as follows: blood clotting in the head and/or around the yolk sac > delay or anomaly in yolk sac absorption > change in swimming equilibrium > development of pericardial and/or yolk sac oedema > scoliosis. A PCR-DGGE analysis was used to evaluate changes in the structure, richness, evenness and diversity of bacterial communities after herbicide exposure. A herbicide-induced structural adjustment of bacterial community was observed. In this study, it was successfully demonstrated that Basagran® affected zebrafish embryos and associated bacterial communities, showing time-dependent and concentration-dependent embryos' developmental response and structural changes in bacterial community. Thus, this work provides for the first time a complementary approach, which is useful to derive robust toxicity thresholds considering the embryo-microbiota system as a whole. The aquatic hazard assessment will be strengthened by combining current ecotoxicological tests with molecular microbiology tools.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping