ZFIN ID: ZDB-PUB-161204-1
The Zebrafish as a Model for Human Bacterial Infections
Neely, M.N.
Date: 2017
Source: Methods in molecular biology (Clifton, N.J.)   1535: 245-266 (Chapter)
Registered Authors: Neely, Melody N.
Keywords: Animal model, Histology, Infection, Microinjection, Pathogenesis, Streptococcus infection, Zebrafish
MeSH Terms:
  • Animals
  • Bacterial Infections/microbiology*
  • Bacterial Infections/pathology
  • Biopsy
  • Disease Models, Animal
  • Humans
  • Immunohistochemistry
  • Larva
  • Microinjections
  • Microscopy, Fluorescence/methods
  • Streptococcal Infections/microbiology
  • Streptococcal Infections/pathology
  • Zebrafish/microbiology*
PubMed: 27914084 Full text @ Meth. Mol. Biol.
The development of the zebrafish (Danio rerio) infectious disease model has provided new insights and information into pathogenesis. Many of these new discoveries would not have been possible using a typical mammalian model. The advantages of using this model are many and in the last 15 years the model has been exploited for the analysis of many different pathogens. Here, we describe in detail how to perform a bacterial infection using either the adult zebrafish or zebrafish larvae using microinjection. Multiple methods of analysis are described that can be used to address specific questions pertaining to disease progression and the interactions with the immune system.