PUBLICATION
Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model.
- Authors
- Liverani, C., La Manna, F., Groenewoud, A., Mercatali, L., Van Der Pluijm, G., Pieri, F., Cavaliere, D., De Vita, A., Spadazzi, C., Miserocchi, G., Bongiovanni, A., Recine, F., Riva, N., Amadori, D., Tasciotti, E., Snaar-Jagalska, E., Ibrahim, T.
- ID
- ZDB-PUB-161130-8
- Date
- 2017
- Source
- Biology Open 6(2): 133-140 (Journal)
- Registered Authors
- Snaar-Jagalska, Ewa B.
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 27895047 Full text @ Biol. Open
Citation
Liverani, C., La Manna, F., Groenewoud, A., Mercatali, L., Van Der Pluijm, G., Pieri, F., Cavaliere, D., De Vita, A., Spadazzi, C., Miserocchi, G., Bongiovanni, A., Recine, F., Riva, N., Amadori, D., Tasciotti, E., Snaar-Jagalska, E., Ibrahim, T. (2017) Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model.. Biology Open. 6(2):133-140.
Abstract
Patient-derived specimens are an invaluable resource to investigate tumor biology. However, in vivo studies on primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized in vitro for their morphological features, sensitivity to drugs and biomarker expression, and in vivo for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of ex vivo cells, and highlights the potential of the zebrafish model to provide a versatile in vivo system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping