PUBLICATION
Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish
- Authors
- Frisca, F., Colquhoun, D., Goldshmit, Y., Änkö, M.L., Pébay, A., Kaslin, J.
- ID
- ZDB-PUB-161125-1
- Date
- 2016
- Source
- Scientific Reports 6: 37678 (Journal)
- Registered Authors
- Goldshmit, Yona, Kaslin, Jan
- Keywords
- Embryology, Lipid signalling
- MeSH Terms
-
- Animals
- Body Patterning*
- Cell Movement
- Cilia/metabolism
- Embryo, Nonmammalian/enzymology*
- Embryonic Development/genetics
- Gene Expression Regulation, Developmental
- Organogenesis
- Phenotype
- Phosphoric Diester Hydrolases/genetics
- Phosphoric Diester Hydrolases/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- rho GTP-Binding Proteins/metabolism
- rho-Associated Kinases/metabolism
- PubMed
- 27883058 Full text @ Sci. Rep.
Citation
Frisca, F., Colquhoun, D., Goldshmit, Y., Änkö, M.L., Pébay, A., Kaslin, J. (2016) Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish. Scientific Reports. 6:37678.
Abstract
Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer's vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1-3 Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping