ZFIN ID: ZDB-PUB-161125-1
Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish
Frisca, F., Colquhoun, D., Goldshmit, Y., Änkö, M.L., Pébay, A., Kaslin, J.
Date: 2016
Source: Scientific Reports   6: 37678 (Journal)
Registered Authors: Goldshmit, Yona, Kaslin, Jan
Keywords: Embryology, Lipid signalling
MeSH Terms:
  • Animals
  • Body Patterning*
  • Cell Movement
  • Cilia/metabolism
  • Embryo, Nonmammalian/enzymology*
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental
  • Organogenesis
  • Phenotype
  • Phosphoric Diester Hydrolases/genetics
  • Phosphoric Diester Hydrolases/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • rho GTP-Binding Proteins/metabolism
  • rho-Associated Kinases/metabolism
PubMed: 27883058 Full text @ Sci. Rep.
Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer's vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1-3 Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.