ZFIN ID: ZDB-PUB-161122-9
Parkinson's disease-like motor and non-motor symptoms in rotenone-treated zebrafish
Wang, Y., Liu, W., Yang, J., Wang, F., Yizhen, S., Zhong, Z.M., Wang, H., Hu, L.F., Liu, C.F.
Date: 2017
Source: Neurotoxicology   58: 103-109 (Journal)
Registered Authors: Liu, Chun-Feng, Liu, Wenwen, Wang, Fen, Wang, Han, Wang, Yali
Keywords: Parkinson’s disease, non-motor symptoms, rotenone, zebrafish
MeSH Terms:
  • Animals
  • Anxiety/etiology*
  • Dark Adaptation/drug effects
  • Disease Models, Animal
  • Gene Expression Regulation/drug effects
  • Insecticides/toxicity*
  • Locomotion/drug effects
  • Male
  • Neurotransmitter Agents/metabolism
  • Olfaction Disorders/etiology*
  • Parkinson Disease, Secondary/chemically induced*
  • Parkinson Disease, Secondary/complications*
  • RNA, Messenger/metabolism
  • Rotenone/toxicity*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 27866991 Full text @ Neurotoxicology
The pesticide rotenone is widely used to produce Parkinson's disease (PD)-like symptoms in rodents, but few studies have examined whether rotenone-treated zebrafish can serve as an animal model of PD. Here, we report that 4 weeks of rotenone treatment induced motor and non-motor PD-like symptoms in adult zebrafish. Compared with control fish, rotenone-treated fish spent less time swimming at a fast speed, indicating a deficit in motor function. In the light-dark box test, rotenone-treated fish exhibited longer latencies to enter the dark compartment and spent more time in the light compartment, reflecting anxiety- and depression-like behavior. Furthermore, rotenone-treated fish showed less of an olfactory preference for amino acid, indicating olfactory dysfunction. These behavioral symptoms were associated with decreased levels of dopamine in the brains of rotenone-treated fish. Taken together, these results suggest that rotenone-treated zebrafish are a suitable model of PD.