PUBLICATION

4D holographic microscopy of zebrafish larvae microcirculation

Authors
Donnarumma, D., Brodoline, A., Alexandre, D., Gross, M.
ID
ZDB-PUB-161120-12
Date
2016
Source
Optics express   24: 26887-26900 (Journal)
Registered Authors
Alexandre, Daniel
Keywords
none
MeSH Terms
  • Algorithms
  • Animals
  • Erythrocytes*
  • Holography/methods*
  • Imaging, Three-Dimensional/methods
  • Larva
  • Lighting
  • Microcirculation*
  • Microscopy/methods*
  • Zebrafish
PubMed
27857417 Full text @ Opt. Express
Abstract
An original technique that combines digital holography, dual illumination of the sample and cleaning algorithm 3D reconstruction is proposed. It uses a standard transmission microscopy setup coupled with a digital holography detection. The technique is 4D, since it allows to determine, at each time step, the 3D locations (x,y,z) of many moving objects that scatter the dual illumination beam. The technique has been validated by imaging the microcirculation of blood in a fish larvae sample (the moving objects are thus red blood cells RBCs). Videos showing in 4D the moving RBCs superimposed with the perfused blood vessels are obtained.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes