PUBLICATION

Feeding of nano scale oats β-glucan enhances the host resistance against Edwardsiella tarda and protective immune modulation in zebrafish larvae

Authors
Udayangani, R.M., Dananjaya, S.H., Fronte, B., Kim, C.H., Lee, J., De Zoysa, M.
ID
ZDB-PUB-161120-1
Date
2017
Source
Fish & shellfish immunology   60: 72-77 (Journal)
Registered Authors
Kim, Cheol-Hee
Keywords
Bio-degradable, Edwardsiella tarda, Immunostimulant, Nano-scale beta glucan (NBG)
MeSH Terms
  • Adjuvants, Immunologic
  • Animals
  • Avena/chemistry*
  • Dietary Supplements*
  • Edwardsiella tarda/physiology
  • Enterobacteriaceae Infections/immunology
  • Enterobacteriaceae Infections/veterinary*
  • Fish Diseases/immunology*
  • Nanoparticles
  • Real-Time Polymerase Chain Reaction/veterinary
  • Up-Regulation/immunology
  • Zebrafish/immunology*
  • beta-Glucans/administration & dosage
  • beta-Glucans/immunology*
PubMed
27864158 Full text @ Fish Shellfish Immunol.
Abstract
In this study, we prepared and characterized the oats origin of nano scale β-glucan (NBG) and investigated the immunomodulatory properties in zebrafish larvae. Newly prepared NBG (average particle size of 465 nm) was fully soluble in water. Zebrafish larvae survival rate was increased against pathogenic bacteria Edwardsiella tarda, when NBG was added to the water (500 μg/mL) compared to NBG non-exposed controls. Moreover, quantitative real time PCR (qRT-PCR) results showed up-regulation of immune functional genes including TNF-α, IL-1β, β-defensin, lysozyme, IL 10, IL 12 and C-Rel indicating higher survival rate could be due to stronger immunomodulatory function of NBG (500 μg/mL). Thus, non-toxic, water soluble and biodegradable NBG from oats could be considered as the potential immunostimulant for larval aquaculture.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping