PUBLICATION

Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration

Authors
Tornini, V.A., Puliafito, A., Slota, L.A., Thompson, J.D., Nachtrab, G., Kaushik, A.L., Kapsimali, M., Primo, L., Di Talia, S., Poss, K.D.
ID
ZDB-PUB-161115-7
Date
2016
Source
Current biology : CB   26(22): 2981-2991 (Journal)
Registered Authors
Kapsimali, Marika, Nachtrab, Greg, Poss, Kenneth D., Thompson, John D., Tornini, Valerie A.
Keywords
appendage, blastema, clonal analysis, fibroblast, fin, positional identity, regeneration, tph1b, vertebrate, zebrafish
MeSH Terms
  • Female
  • Zebrafish/physiology*
  • Animal Fins/physiology*
  • Male
  • Animals
  • Regeneration*
  • Stem Cells
  • Zebrafish Proteins/metabolism
  • Calcineurin/metabolism
(all 9)
PubMed
27839971 Full text @ Curr. Biol.
Abstract
The blastema is a mass of progenitor cells that enables regeneration of amputated salamander limbs or fish fins. Methodology to label and track blastemal cell progeny has been deficient, restricting our understanding of appendage regeneration. Here, we created a system for clonal analysis and quantitative imaging of hundreds of blastemal cells and their respective progeny in living adult zebrafish undergoing fin regeneration. Amputation stimulates resident cells within a limited recruitment zone to reset proximodistal (PD) positional information and assemble the blastema. Within the newly formed blastema, the spatial coordinates of connective tissue progenitors are predictive of their ultimate contributions to regenerated skeletal structures, indicating early development of an approximate PD pre-pattern. Calcineurin regulates size recovery by controlling the average number of progeny divisions without disrupting this pre-pattern. Our longitudinal clonal analyses of regenerating zebrafish fins provide evidence that connective tissue progenitors are rapidly organized into a scalable blueprint of lost structures.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cz1701TgTransgenic Insertion
    pd49TgTransgenic Insertion
      pd250TgTransgenic Insertion
        s928TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          No data available
          Antibodies
          Orthology
          Engineered Foreign Genes
          Marker Marker Type Name
          CFPEFGCFP
          CreEFGCre
          DsRedEFGDsRed
          EGFPEFGEGFP
          mCherryEFGmCherry
          RFPEFGRFP
          YFPEFGYFP
          1 - 7 of 7
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          Mapping
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