PUBLICATION
Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism
- Authors
- Gómez-Canela, C., Prats, E., Piña, B., Tauler, R.
- ID
- ZDB-PUB-161115-6
- Date
- 2017
- Source
- Environmental pollution (Barking, Essex : 1987) 220(Pt B): 1231-1243 (Journal)
- Registered Authors
- Piña, Benjamin
- Keywords
- Environmental pollution, LC-HRMS, Metabolomics, ROI-MCR-ALS, Zebrafish
- MeSH Terms
-
- Animals
- Chlorpyrifos/toxicity*
- Chromatography, Liquid
- Insecticides/toxicity*
- Least-Squares Analysis
- Male
- Mass Spectrometry
- Muscles/drug effects*
- Muscles/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- PubMed
- 27839997 Full text @ Environ. Pollut.
Citation
Gómez-Canela, C., Prats, E., Piña, B., Tauler, R. (2017) Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism. Environmental pollution (Barking, Essex : 1987). 220(Pt B):1231-1243.
Abstract
In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping