PUBLICATION

Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos

Authors
Doganli, C., Sandoval, M., Thomas, S., Hart, D.
ID
ZDB-PUB-161111-1
Date
2017
Source
Methods in molecular biology (Clifton, N.J.)   1507: 59-66 (Chapter)
Registered Authors
Doganli, Canan
Keywords
ATAC-seq, Chromatin accessibility, Genome wide, Zebrafish
MeSH Terms
  • Animals
  • Chromatin/isolation & purification
  • Embryo, Nonmammalian
  • Genome
  • High-Throughput Nucleotide Sequencing*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Transposases/chemistry
  • Zebrafish/genetics*
PubMed
27832532 Full text @ Meth. Mol. Biol.
Abstract
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) is a useful method to map genome-wide chromatin accessibility and nucleosome positioning. Genome-wide sequencing is performed utilizing adapter sequences inserted by a prokaryotic transposase, Tn5, into the accessible regions of chromatin. Here we describe the use of ATAC-seq in the zebrafish embryo and thereby the applicability of this approach in whole vertebrate embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping