PUBLICATION
Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos
- Authors
- Doganli, C., Sandoval, M., Thomas, S., Hart, D.
- ID
- ZDB-PUB-161111-1
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1507: 59-66 (Chapter)
- Registered Authors
- Doganli, Canan
- Keywords
- ATAC-seq, Chromatin accessibility, Genome wide, Zebrafish
- MeSH Terms
-
- Animals
- Chromatin/isolation & purification
- Embryo, Nonmammalian
- Genome
- High-Throughput Nucleotide Sequencing*
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Transposases/chemistry
- Zebrafish/genetics*
- PubMed
- 27832532 Full text @ Meth. Mol. Biol.
Citation
Doganli, C., Sandoval, M., Thomas, S., Hart, D. (2017) Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 1507:59-66.
Abstract
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) is a useful method to map genome-wide chromatin accessibility and nucleosome positioning. Genome-wide sequencing is performed utilizing adapter sequences inserted by a prokaryotic transposase, Tn5, into the accessible regions of chromatin. Here we describe the use of ATAC-seq in the zebrafish embryo and thereby the applicability of this approach in whole vertebrate embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping