PUBLICATION
Image resolution and deconvolution in optical tomography
- Authors
- van der Horst, J., Kalkman, J.
- ID
- ZDB-PUB-161110-4
- Date
- 2016
- Source
- Optics express 24: 24460-24472 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 27828174 Full text @ Opt. Express
Citation
van der Horst, J., Kalkman, J. (2016) Image resolution and deconvolution in optical tomography. Optics express. 24:24460-24472.
Abstract
We present a frequency domain analysis of the image resolution of optical tomography systems. The result of our analysis is a description of the spatially-variant resolution in optical tomographic image after reconstruction as a function of the properties of the imaging system geometry. We validate our model using optical projection tomography (OPT) measurements of fluorescent beads embedded in agarose gel. Our model correctly describes both the radial and tangential resolution of the measured images. In addition, we present a correction of the tomographic images for the spatially-varying resolution using a deconvolution algorithm. The resulting corrected tomographic reconstruction shows a homogeneous and isotropic pixel-limited resolution across the entire image. Our method is applied to OPT measurements of a zebrafish, showing improved resolution. Aside from allowing image correction and providing a resolution measure for OPT systems, our model provides a powerful tool for the design of optical tomographic systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping