PUBLICATION
Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope
- Authors
- Wilding, D., Pozzi, P., Soloviev, O., Vdovin, G., Verhaegen, M.
- ID
- ZDB-PUB-161110-3
- Date
- 2016
- Source
- Optics express 24: 24896-24906 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Fluorescence
- Microscopy, Fluorescence*
- Optics and Photonics
- Zebrafish
- PubMed
- 27828430 Full text @ Opt. Express
Citation
Wilding, D., Pozzi, P., Soloviev, O., Vdovin, G., Verhaegen, M. (2016) Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope. Optics express. 24:24896-24906.
Abstract
A methodology for the adaptive control and correction of phase aberrations in the illumination arm of a light-sheet fluorescence microscope has been developed. The method uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the sample. Using this signal, the aberrations in the illumination arm are subsequently corrected with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in significant improvement in the axial resolution, has been demonstrated by imaging Tg(fli:GFP) zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping