ZFIN ID: ZDB-PUB-161031-1
Who knows not where an anemone does wear his sting? Could polypeptides released from the columnar vesicles of Bunodosoma cangicum induce apoptosis in the ZF-L cell line?
Bastos, C.L., Varela, A.S., Ferreira, S.P., Nornberg, B.F., Boyle, R.T.
Date: 2016
Source: Toxicon : official journal of the International Society on Toxinology   124: 73-82 (Journal)
Registered Authors: Nornberg, Bruna Felix
Keywords: Anemone venom, Apoptosis, Columnar vesicles, Zebrafish cell line
MeSH Terms:
  • Animals
  • Apoptosis/drug effects*
  • Cell Line
  • Chromatography, Gel
  • Cnidarian Venoms/pharmacology*
  • Electrophoresis, Polyacrylamide Gel
  • Microscopy, Electron, Scanning
  • Peptides/metabolism*
  • Sea Anemones/metabolism*
  • Zebrafish
PubMed: 27794434 Full text @ Toxicon.
We provide ultrastructural and cytological evidence that the tentacles of the sea anemone Bunodosoma cangicum does not contain cytotoxic venom. However, we show that the stimulated secretion of an apparent mixture of biomolecules containing polypeptides from the columnar vesicles of Bunodosoma cangicum is apparently a potent inducer of apoptosis in the zebrafish cell line, ZF-L. Microscopic fluorescence, cell morphology and flow cytometric assays confirm the apoptotic activity. Crude vesicle venom was partially purified by size exclusion chromatography. PAGE analysis shows that this venom contains low weight polypeptides but no measurable protein. The apoptotic activity is heat labile, and the observed peptides concurrent with this activity have a molecular weight of approximately 2000 Daltons. This manuscript is the first report of biologically active molecules and peptides associated with columnar vesicles of anemones, and the first to confirm that the tentacles of B. cangicum do not contain cytotoxic venom, and express spirocytes exclusively.