PUBLICATION
Collagen XII Contributes to Epicardial and Connective Tissues in the Zebrafish Heart during Ontogenesis and Regeneration
- Authors
- Marro, J., Pfefferli, C., de Preux Charles, A.S., Bise, T., Ja?wi?ska, A.
- ID
- ZDB-PUB-161027-3
- Date
- 2016
- Source
- PLoS One 11: e0165497 (Journal)
- Registered Authors
- Jazwinska, Anna
- Keywords
- Collagens, Heart regeneration, Zebrafish, Myocardium, Heart, Epicardium, Extracellular matrix, Cardiac ventricles
- MeSH Terms
-
- Receptors, Transforming Growth Factor beta/antagonists & inhibitors
- Receptors, Transforming Growth Factor beta/metabolism
- Benzamides/pharmacology
- Animals, Genetically Modified/metabolism
- Heart/physiology*
- Zebrafish/metabolism
- Connective Tissue/metabolism*
- Vimentin/metabolism
- In Situ Hybridization
- Zebrafish Proteins/metabolism*
- Transforming Growth Factor beta/metabolism
- Heart Ventricles/metabolism
- Heart Ventricles/pathology
- Signal Transduction/drug effects
- Up-Regulation/drug effects
- Collagen Type XII/genetics
- Collagen Type XII/metabolism*
- Myocardium/metabolism
- Myocardium/pathology
- Dioxoles/pharmacology
- Collagen Type I/genetics
- Collagen Type I/metabolism
- Animals
- Regeneration/physiology
- Pericardium/metabolism*
- Pericardium/pathology
- Microscopy, Fluorescence
- PubMed
- 27783651 Full text @ PLoS One
Citation
Marro, J., Pfefferli, C., de Preux Charles, A.S., Bise, T., Ja?wi?ska, A. (2016) Collagen XII Contributes to Epicardial and Connective Tissues in the Zebrafish Heart during Ontogenesis and Regeneration. PLoS One. 11:e0165497.
Abstract
Zebrafish heart regeneration depends on cardiac cell proliferation, epicardium activation and transient reparative tissue deposition. The contribution and the regulation of specific collagen types during the regenerative process, however, remain poorly characterized. Here, we identified that the non-fibrillar type XII collagen, which serves as a matrix-bridging component, is expressed in the epicardium of the zebrafish heart, and is boosted after cryoinjury-induced ventricular damage. During heart regeneration, an intense deposition of Collagen XII covers the outer epicardial cap and the interstitial reparative tissue. Analysis of the activated epicardium and fibroblast markers revealed a heterogeneous cellular origin of Collagen XII. Interestingly, this matrix-bridging collagen co-localized with fibrillar type I collagen and several glycoproteins in the post-injury zone, suggesting its role in tissue cohesion. Using SB431542, a selective inhibitor of the TGF-β receptor, we showed that while the inhibitor treatment did not affect the expression of collagen 12 and collagen 1a2 in the epicardium, it completely suppressed the induction of both genes in the fibrotic tissue. This suggests that distinct mechanisms might regulate collagen expression in the outer heart layer and the inner injury zone. On the basis of this study, we postulate that the TGF-β signaling pathway induces and coordinates formation of a transient collagenous network that comprises fibril-forming Collagen I and fiber-associated Collagen XII, both of which contribute to the reparative matrix of the regenerating zebrafish heart.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping