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ZFIN ID: ZDB-PUB-161014-4
Identification of Germ-Line Stem Cells in Zebrafish
Draper, B.W.
Date: 2017
Source: Methods in molecular biology (Clifton, N.J.)   1463: 103-113 (Chapter)
Registered Authors: Draper, Bruce
Keywords: Confocal immunofluorescence, Fluorescent RNA in situ hybridization, Oogonial stem cell, Spermatogonial stem cell, Vasa, Zebrafish germ-line stem cells, Zebrafish ovary, Zebrafish testis, nanos2
MeSH Terms:
  • Adult Germline Stem Cells/chemistry*
  • Adult Germline Stem Cells/metabolism
  • Animals
  • DEAD-box RNA Helicases/metabolism
  • Female
  • Fluorescent Antibody Technique
  • In Situ Hybridization, Fluorescence
  • Male
  • Oogonial Stem Cells/chemistry*
  • Oogonial Stem Cells/metabolism
  • Ovary/cytology
  • Ovary/metabolism
  • RNA-Binding Proteins/genetics*
  • Testis/cytology
  • Testis/metabolism
  • Tissue Fixation
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 27734351 Full text @ Meth. Mol. Biol.
ABSTRACT
Both male and female zebrafish have a population of germ-line stem cells that produce gametes throughout the life of the fish. These cells localize to specific regions in the gonads and can be identified because they uniquely express the nanos2 gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect nanos2 mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.
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