PUBLICATION
Differentially transcriptional regulation on cell cycle pathway by silver nanoparticles from ionic silver in larval zebrafish (Danio rerio)
- Authors
- Kang, J.S., Bong, J., Choi, J.S., Henry, T.B., Park, J.W.
- ID
- ZDB-PUB-161007-36
- Date
- 2016
- Source
- Biochemical and Biophysical Research Communications 479(4): 753-758 (Journal)
- Registered Authors
- Henry, Theodore B.
- Keywords
- Cell cycle, Global gene expression, Silver ion, Silver nanoparticles, Zebrafish
- MeSH Terms
-
- Animals
- Cations, Monovalent/chemistry
- Cations, Monovalent/toxicity
- Cell Cycle/drug effects*
- Cell Cycle/genetics*
- Gene Expression Regulation/drug effects
- Gene Regulatory Networks/drug effects
- Larva/cytology
- Larva/drug effects
- Larva/genetics
- Metal Nanoparticles/chemistry
- Metal Nanoparticles/toxicity*
- Silver/chemistry
- Silver/toxicity*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 27693782 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kang, J.S., Bong, J., Choi, J.S., Henry, T.B., Park, J.W. (2016) Differentially transcriptional regulation on cell cycle pathway by silver nanoparticles from ionic silver in larval zebrafish (Danio rerio). Biochemical and Biophysical Research Communications. 479(4):753-758.
Abstract
Silver nanoparticles (AgNPs) have a strong antibacterial activity and the relevant modes of actions have regarded as direct or indirect causes of toxicity observed in the environment. In this study, the transcriptomic profiles in larval zebrafish (Danio rerio) exposed to AgNPs (about 50 nm in size) and AgNO3 as a comparative ionic silver were investigated and analyzed using differential expressed gene (DEG), Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses. Results indicated that underlying molecular mechanisms are different each other. Interestingly, the global gene expression profiling showed that cell cycle pathway is affected by both AgNPs and dissolved Ag+, however its regulation pattern was opposite each other. To the best of our knowledge, the up-regulation of cell cycle pathway by AgNPs and down-regulation by Ag+ is the first reporting and suggests the distinguished toxicological perspective from a well-known hypothesis that Ag+ mainly regulates the cell cycle. This study provides novel insights onto the genotoxicological mechanisms of AgNPs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping