PUBLICATION
ERK activation in endothelial cells is a novel marker during neovasculogenesis
- Authors
- Nagasawa-Masuda, A., Terai, K.
- ID
- ZDB-PUB-161007-31
- Date
- 2016
- Source
- Genes to cells : devoted to molecular & cellular mechanisms 21(11): 1164-1175 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Aorta/cytology
- Aorta/embryology
- Endothelial Cells/enzymology*
- Enzyme Activation
- Extracellular Signal-Regulated MAP Kinases/metabolism*
- Neovascularization, Physiologic*
- Veins/cytology
- Veins/embryology
- Zebrafish/embryology
- Zebrafish Proteins/metabolism*
- PubMed
- 27696620 Full text @ Genes Cells
Citation
Nagasawa-Masuda, A., Terai, K. (2016) ERK activation in endothelial cells is a novel marker during neovasculogenesis. Genes to cells : devoted to molecular & cellular mechanisms. 21(11):1164-1175.
Abstract
Vasculogenesis is essential during early development to construct networks transporting oxygen, blood and nutrients. Tip and stalk cells are specialized endothelial cells involved in novel vessel formation because of their behavior such as sprouting as a leading cell and following tip cell. However, the spatiotemporal details determining the emergence of these cells are unknown. Here, we first show that the ERK activity in endothelial cells represents the precursor of tip and stalk cells for vasculogenesis in zebrafish. We identified that tip and stalk cells for intersegmental vessel (ISV) formation were already specialized in the dorsal aorta (DA) before sprouting. Furthermore, similar specialization was observed in tip cells during parachordal vessel (PAV) formation in lymphangiogenesis. We also identified that the ERK activity was required for specialized cells to emerge from existing blood vessels. Our data show that the ERK activity is a novel marker for determining the emergence of cells in both angiogenesis and lymphangiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping