PUBLICATION
Unexpected Neuroprotective Effects of Loganin on 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neurotoxicity and Cell Death in Zebrafish
- Authors
- Yao, L., Peng, S.X., Xu, Y.D., Lin, S.L., Li, Y.H., Liu, C.J., Zhao, H.D., Wang, L.F., Shen, Y.Q.
- ID
- ZDB-PUB-160924-1
- Date
- 2017
- Source
- Journal of cellular biochemistry 118(3): 615-628 (Journal)
- Registered Authors
- Keywords
- MPTP, loganin, neuroprotection, neurotoxicity, zebrafish
- MeSH Terms
-
- 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine*
- Animals
- Iridoids/pharmacology*
- MAP Kinase Kinase 4/metabolism
- MPTP Poisoning/embryology
- MPTP Poisoning/prevention & control*
- Neuroprotective Agents/pharmacology*
- PC12 Cells
- Phosphatidylinositol 3-Kinases/metabolism
- Proto-Oncogene Proteins c-akt/metabolism
- Rats
- TOR Serine-Threonine Kinases/metabolism
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism
- PubMed
- 27662601 Full text @ J. Cell. Biochem.
Citation
Yao, L., Peng, S.X., Xu, Y.D., Lin, S.L., Li, Y.H., Liu, C.J., Zhao, H.D., Wang, L.F., Shen, Y.Q. (2017) Unexpected Neuroprotective Effects of Loganin on 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neurotoxicity and Cell Death in Zebrafish. Journal of cellular biochemistry. 118(3):615-628.
Abstract
1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP), which induces the pathological characteristics of Parkinson's disease in rodents, also specifically targets dopaminergic neurons in zebrafish embryos and larvae. Loganin, a traditional Chinese drug, was reported to regulate immune function and possess anti-inflammatory and anti-shock effects. Here, we investigate the role of loganin in MPTP-induced Parkinson-like abnormalities in zebrafish. MPTP treatment induced abnormal development, in larvae, such as pericardium edema, increased yolk color, yolk sac edema, and retarded yolk sac resorption, as well as defects in brain development. Loganin could block MPTP-induced defects, with little toxicity to the eggs. Results of whole mount in situ hybridization showed loganin prevented the loss of both dopaminergic neurons and locomotor activity, exhibited by larvae treated with MPTP. In addition, loganin significantly rescued MPTP-induced neurotoxicity on PC12 cells, possibly through the suppression of PI3K/Akt/mTOR axis and JNK signaling pathways. In conclusion, loganin blocks MPTP-induced neurotoxicity and abnormal development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping