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ZIRC
ZFIN ID: ZDB-PUB-160915-7
Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish
Martella, A., Sepe, R.M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S.C., Sordino, P., Di Marzo, V.
Date: 2016
Source: FASEB journal : official publication of the Federation of American Societies for Experimental Biology   30(12): 4275-4288 (Journal)
Registered Authors: Carnevali, Oliana, Neuhauss, Stephan, Sordino, Paolo, Zang, Jingjing
Keywords: 2-arachidonoylglycerol, CB1, DAGL, MAGL, axonal pathfinding
MeSH Terms:
  • Animals
  • Axons/metabolism*
  • Axons/ultrastructure
  • Behavior, Animal/physiology
  • Brain/metabolism*
  • Endocannabinoids/metabolism*
  • Lipoprotein Lipase/metabolism*
  • Locomotion/physiology*
  • Signal Transduction*/physiology
  • Zebrafish
PubMed: 27623930 Full text @ FASEB J.
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ABSTRACT
The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report the presence of a complete endocannabinoid system during zebrafish development and show that the genes that code for enzymes that catalyze the anabolism and catabolism (mgll and dagla) of the endocannabinoid, 2-AG (2-arachidonoylglycerol), as well as 2-AG main receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino-induced transient knockdown of the zebrafish Daglα homolog and its pharmacologic rescue, we suggest that synthesis of 2-AG is implicated in the control of axon formation in the midbrain-hindbrain region and that animals that lack Daglα display abnormal physiological behaviors in tests that measure stereotyped movement and motion perception. Our results suggest that the well-established role for 2-AG in axonal outgrowth has implications for the control of vision and movement in zebrafish and, thus, is likely common to all vertebrates.-Martella, A., Sepe, R. M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S. C. F., Sordino, P., Di Marzo, V. Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish.
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