PUBLICATION
Novel O-GlcNAcylation on Ser(40) of canonical H2A isoforms specific to viviparity
- Authors
- Hirosawa, M., Hayakawa, K., Yoneda, C., Arai, D., Shiota, H., Suzuki, T., Tanaka, S., Dohmae, N., Shiota, K.
- ID
- ZDB-PUB-160913-12
- Date
- 2016
- Source
- Scientific Reports 6: 31785 (Journal)
- Registered Authors
- Keywords
- Epigenetics, Molecular evolution
- MeSH Terms
-
- Acetylglucosamine/chemistry*
- Animals
- Antibodies, Monoclonal/chemistry
- Binding Sites
- Biological Evolution*
- Bombyx
- Cattle
- Chromatography, High Pressure Liquid
- Embryonic Stem Cells/cytology
- HeLa Cells
- Histones/chemistry*
- Humans
- Macaca
- Mass Spectrometry
- Mice
- Mice, Inbred C57BL
- Phylogeny
- Protein Isoforms/chemistry
- Recombinant Proteins/chemistry
- Serine/chemistry
- Species Specificity
- Trophoblasts/metabolism
- Xenopus
- Zebrafish
- PubMed
- 27615797 Full text @ Sci. Rep.
Citation
Hirosawa, M., Hayakawa, K., Yoneda, C., Arai, D., Shiota, H., Suzuki, T., Tanaka, S., Dohmae, N., Shiota, K. (2016) Novel O-GlcNAcylation on Ser(40) of canonical H2A isoforms specific to viviparity. Scientific Reports. 6:31785.
Abstract
We report here newly discovered O-linked-N-acetylglucosamine (O-GlcNAc) modification of histone H2A at Ser(40) (H2AS40Gc). The mouse genome contains 18 H2A isoforms, of which 13 have Ser(40) and the other five have Ala(40). The combination of production of monoclonal antibody and mass spectrometric analyses with reverse-phase (RP)-high performance liquid chromatography (HPLC) fractionation indicated that the O-GlcNAcylation is specific to the Ser(40) isoforms. The H2AS40Gc site is in the L1 loop structure where two H2A molecules interact in the nucleosome. Targets of H2AS40Gc are distributed genome-wide and are dramatically changed during the process of differentiation in mouse trophoblast stem cells. In addition to the mouse, H2AS40Gc was also detected in humans, macaques and cows, whereas non-mammalian species possessing only the Ala(40) isoforms, such as silkworms, zebrafish and Xenopus showed no signal. Genome database surveys revealed that Ser(40) isoforms of H2A emerged in Marsupialia and persisted thereafter in mammals. We propose that the emergence of H2A Ser(40) and its O-GlcNAcylation linked a genetic event to genome-wide epigenetic events that correlate with the evolution of placental animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping