PUBLICATION
Steering cell migration by alternating blebs and actin-rich protrusions
- Authors
- Diz-Muņoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., Heisenberg, C.P., Paluch, E.K.
- ID
- ZDB-PUB-160904-4
- Date
- 2016
- Source
- BMC Biology 14: 74 (Journal)
- Registered Authors
- Heisenberg, Carl-Philipp
- Keywords
- Actin-rich protrusion, Bleb, Cell migration, Directionality, Persistence, Protrusion orientation, Run and tumble
- MeSH Terms
-
- Endoderm/cytology
- Morpholinos/pharmacology
- Animals
- Cell Movement*/drug effects
- Mesoderm/cytology
- Pseudopodia/drug effects
- Pseudopodia/metabolism*
- Zebrafish/metabolism*
- Stem Cells/cytology
- Stem Cells/drug effects
- Stem Cells/metabolism
- Actins/metabolism*
- PubMed
- 27589901 Full text @ BMC Biol.
Citation
Diz-Muņoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., Heisenberg, C.P., Paluch, E.K. (2016) Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 14:74.
Abstract
Background High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood.
Results Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision.
Conclusions Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times.
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