PUBLICATION
Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos
- Authors
- Tseng, L.C., Tang, C.H., Jiang, Y.J.
- ID
- ZDB-PUB-160902-10
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1470: 213-24 (Chapter)
- Registered Authors
- Jiang, Yun-Jin, Tang, Chih-Hao, Tseng, Li-Chuan
- Keywords
- In situ hybridization, Knockdown, Morpholinos, RNA probe, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Gastrulation/genetics
- Gene Expression Regulation, Developmental*
- Gene Knockdown Techniques/methods
- High-Throughput Screening Assays/methods*
- In Situ Hybridization/methods*
- Microinjections/methods
- Morpholinos/genetics*
- Morpholinos/pharmacology
- Ubiquitin-Specific Proteases/genetics
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 27581296 Full text @ Meth. Mol. Biol.
Citation
Tseng, L.C., Tang, C.H., Jiang, Y.J. (2016) Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 1470:213-24.
Abstract
High-throughput screening with a loss-of-function strategy is a logical and efficient way to identify novel genes involved in biological processes of interest. In zebrafish, morpholinos have been developed as a convenient tool to knock down gene expression. Here, we describe procedures for systematic screening using morpholinos in zebrafish to identify novel deubiquitylases involved in convergent extension during gastrulation. In this example, we examine candidates based on embryonic morphology and molecular signals of whole mount in situ hybridization assay.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping