PUBLICATION
Maternal exposure to the water soluble fraction of crude oil, lead and their mixture induces autism-like behavioral deficits in zebrafish (Danio rerio) larvae
- Authors
- Wang, Y., Zhong, H., Wang, C., Gao, D., Zhou, Y., Zuo, Z.
- ID
- ZDB-PUB-160831-5
- Date
- 2016
- Source
- Ecotoxicology and environmental safety 134P1: 23-30 (Journal)
- Registered Authors
- Keywords
- Aquatic models, Autism spectrum disorder, Behavioral testing, Lead, Water soluble fraction of crude oil
- MeSH Terms
- none
- PubMed
- 27573365 Full text @ Ecotoxicol. Environ. Saf.
Citation
Wang, Y., Zhong, H., Wang, C., Gao, D., Zhou, Y., Zuo, Z. (2016) Maternal exposure to the water soluble fraction of crude oil, lead and their mixture induces autism-like behavioral deficits in zebrafish (Danio rerio) larvae. Ecotoxicology and environmental safety. 134P1:23-30.
Abstract
Autism spectrum disorder (ASD) is a serious debilitating mental illness with complex symptoms and multi-factorial pathogenesis. Although the pathogenesis of ASD remains unclear, etiology is thought to involve complex, multigenic interactions and possible environmental contributions. In the present study, we used zebrafish (Danio rerio) as a model to investigate whether maternal exposure to the water soluble fraction of crude oil (WSF, 5μg/L), lead (Pb, 20μg/L) and their mixture (5 μg/L WSF+20 μg/L Pb) could induce autism-like behavior in larvae. Our results showed that isolated and combined WSF/Pb exposure altered the behavioral pattern of fish swimming. WSF significantly increased anxiety and locomotor activity, decreased repetitive behavior in the open field test, and reduced the level of serotonin. However, co-exposure to WSF/Pb decreased behavioral activity and shoaling behavior, and increased cycle swimming and edge preference. Significant changes in the expression level of the multiple genes potentially critical for regulating environmental factor induced autism-like behavior were found. A gene network regulating ASD disturbed by WSF/Pb exposure was established using computational analysis. The information from the network could provide a clue for further mechanistic studies explaining molecular events regulating WSF/Pb mediated ASD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping