PUBLICATION
Expression profiling identifies Sertoli and Leydig cell genes as Fsh targets in adult zebrafish testis
- Authors
- Crespo, D., Assis, L.H., Furmanek, T., Bogerd, J., Schulz, R.W.
- ID
- ZDB-PUB-160828-3
- Date
- 2016
- Source
- Molecular and Cellular Endocrinology 437: 237-251 (Journal)
- Registered Authors
- Bogerd, Jan, Schulz, Rüdiger W.
- Keywords
- Follicle-stimulating hormone, RNA sequencing, Spermatogenesis, Zebrafish
- Datasets
- GEO:GSE84436
- MeSH Terms
-
- Signal Transduction/drug effects
- Testis/drug effects
- Testis/metabolism*
- Spermatogonia/cytology
- Spermatogonia/drug effects
- Spermatogonia/metabolism
- Gene Expression Regulation, Developmental/drug effects*
- Leydig Cells/cytology
- Leydig Cells/drug effects
- Leydig Cells/metabolism*
- Follicle Stimulating Hormone/pharmacology*
- Male
- Transcriptome/genetics
- Humans
- Gene Expression Profiling*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Androgens/metabolism
- HEK293 Cells
- Recombinant Proteins/pharmacology
- Sertoli Cells/cytology
- Sertoli Cells/drug effects
- Sertoli Cells/metabolism*
- Aging/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Cell Proliferation/genetics
- Protein Interaction Maps
- Spermatogenesis/drug effects
- Spermatogenesis/genetics
- Animals
- Zebrafish/genetics*
- Zebrafish/growth & development*
- PubMed
- 27566230 Full text @ Mol. Cell. Endocrinol.
Citation
Crespo, D., Assis, L.H., Furmanek, T., Bogerd, J., Schulz, R.W. (2016) Expression profiling identifies Sertoli and Leydig cell genes as Fsh targets in adult zebrafish testis. Molecular and Cellular Endocrinology. 437:237-251.
Abstract
Spermatogonial stem cells are quiescent, undergo self-renewal or differentiating divisions, thereby forming the cellular basis of spermatogenesis. This cellular development is orchestrated by follicle-stimulating hormone (FSH), through the production of Sertoli cell-derived factors, and by Leydig cell-released androgens. Here, we investigate the transcriptional events induced by Fsh in a steroid-independent manner on the restart of zebrafish (Danio rerio) spermatogenesis ex vivo, using testis from adult males where type A spermatogonia were enriched by estrogen treatment in vivo. Under these conditions, RNA sequencing preferentially detected differentially expressed genes in somatic/Sertoli cells. Fsh-stimulated spermatogonial proliferation was accompanied by modulating several signaling systems (i.e. Tgf-β, Hedgehog, Wnt and Notch pathways). In silico protein-protein interaction analysis indicated a role for Hedgehog family members potentially integrating signals from different pathways during fish spermatogenesis. Moreover, Fsh had a marked impact on metabolic genes, such as lactate and fatty acid metabolism, or on Sertoli cell barrier components. Fish Leydig cells express the Fsh receptor and one of the most robust Fsh-responsive genes was insulin-like 3 (insl3), a Leydig cell-derived growth factor. Follow-up work showed that recombinant zebrafish Insl3 mediated pro-differentiation effects of Fsh on spermatogonia in an androgen-independent manner. Our experimental approach allowed focusing on testicular somatic genes in zebrafish and showed that the activity of signaling systems known to be relevant in stem cell systems was modulated by Fsh, providing promising leads for future work, as exemplified by the studies on Insl3.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping