Autotaxin-Lysophosphatidic Acid Axis Acts Downstream of Apoprotein B Lipoproteins in Endothelial Cells

Gibbs-Bar, L., Tempelhof, H., Ben-Hamo, R., Ely, Y., Brandis, A., Hofi, R., Almog, G., Braun, T., Feldmesser, E., Efroni, S., Yaniv, K.
Arteriosclerosis, Thrombosis, and Vascular Biology   36(10): 2058-67 (Journal)
Registered Authors
Almog, Gabriella, Ely, Yona, Hofi, Roy, Tempelhof, Hanoch, Yaniv, Karina
apoprotein, cardiovascular disease, lipoproteins, lysophosphatidic acid, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Apolipoproteins B/blood
  • Apolipoproteins B/genetics
  • Apolipoproteins B/metabolism*
  • Cell Proliferation
  • Cells, Cultured
  • Disease Models, Animal
  • Endothelial Cells/enzymology*
  • Gene Expression Profiling/methods
  • Genotype
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics
  • Human Umbilical Vein Endothelial Cells/enzymology
  • Humans
  • Hyperlipidemias/blood
  • Hyperlipidemias/enzymology*
  • Hyperlipidemias/genetics
  • Lysophospholipids/blood
  • Lysophospholipids/metabolism*
  • Mutation
  • Neovascularization, Physiologic*
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Phosphoric Diester Hydrolases/blood
  • Phosphoric Diester Hydrolases/genetics
  • Phosphoric Diester Hydrolases/metabolism*
  • Receptors, Lysophosphatidic Acid/metabolism
  • Signal Transduction
  • Transcription Factors/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/blood
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
27562917 Full text @ Arterio., Thromb., and Vas. Bio.
As they travel through the blood stream, plasma lipoproteins interact continuously with endothelial cells (ECs). Although the focus of research has mostly been guided by the importance of lipoproteins as risk factors for atherosclerosis, thrombosis, and other cardiovascular diseases, little is known about the mechanisms linking lipoproteins and angiogenesis under physiological conditions, and particularly, during embryonic development. In this work, we performed global mRNA expression profiling of endothelial cells from hypo-, and hyperlipidemic zebrafish embryos with the goal of uncovering novel mediators of lipoprotein signaling in the endothelium.
Microarray analysis was conducted on FACS-isolated fli1:EGFP(+) ECs from normal, hypo-, and hyperlipidemic zebrafish embryos. We found that opposed levels of apoprotein B lipoproteins result in differential expression of the secreted enzyme autotaxin in ECs, which in turn affects EC sprouting and angiogenesis. We further demonstrate that the effects of autotaxin in vivo are mediated by lysophosphatidic acid-a well-known autotaxin activity product-and that LPA and LPA receptors participate as well in the response of ECs to lipoprotein levels.
Our findings provide the first in vivo gene expression profiling of ECs facing different levels of plasma apoprotein B lipoproteins and uncover a novel lipoprotein-autotaxin-LPA axis as regulator of EC behavior. These results highlight new roles for lipoproteins as signaling molecules, which are independent of their canonical function as cholesterol transporters.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes