PUBLICATION
Intravital assessment of myelin molecular order with polarimetric multiphoton microscopy
- Authors
- Turcotte, R., Rutledge, D.J., Bélanger, E., Dill, D., Macklin, W.B., Côté, D.C.
- ID
- ZDB-PUB-160820-11
- Date
- 2016
- Source
- Scientific Reports 6: 31685 (Journal)
- Registered Authors
- Macklin, Wendy B.
- Keywords
- Biophysics, Medical research, Microscopy, Myelin biology and repair, Optical physics
- MeSH Terms
-
- Zebrafish/embryology*
- Myelin Sheath/metabolism*
- Animals
- Intravital Microscopy/methods*
- Microscopy, Fluorescence, Multiphoton/methods*
- PubMed
- 27538357 Full text @ Sci. Rep.
Citation
Turcotte, R., Rutledge, D.J., Bélanger, E., Dill, D., Macklin, W.B., Côté, D.C. (2016) Intravital assessment of myelin molecular order with polarimetric multiphoton microscopy. Scientific Reports. 6:31685.
Abstract
Myelin plays an essential role in the nervous system and its disruption in diseases such as multiple sclerosis may lead to neuronal death, thus causing irreversible functional impairments. Understanding myelin biology is therefore of fundamental and clinical importance, but no tools currently exist to describe the fine spatial organization of myelin sheaths in vivo. Here we demonstrate intravital quantification of the myelin molecular structure using a microscopy method based on polarization-resolved coherent Raman scattering. Developmental myelination was imaged noninvasively in live zebrafish. Longitudinal imaging of individual axons revealed changes in myelin organization beyond the diffraction limit. Applied to promyelination drug screening, the method uniquely enabled the identification of focal myelin regions with differential architectures. These observations indicate that the study of myelin biology and the identification of therapeutic compounds will largely benefit from a method to quantify the myelin molecular organization in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping