PUBLICATION
Zebrafish embryo tolerance to environmental stress factors - concentration/dose response analysis of oxygen limitation, pH and UV-light irradiation
- Authors
- Andrade, T.S., Henriques, J.F., Almeida, A.R., Soares, A.M., Scholz, S., Domingues, I.
- ID
- ZDB-PUB-160818-10
- Date
- 2017
- Source
- Environmental toxicology and chemistry 36(3): 682-690 (Journal)
- Registered Authors
- Soares, Amadeu
- Keywords
- Abiotic factors, Danio rerio, Dose response modelling, Global climate change, Stressors
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Drug Tolerance
- Embryo, Nonmammalian*/drug effects
- Embryo, Nonmammalian*/radiation effects
- Hydrogen-Ion Concentration
- Lethal Dose 50
- Oxygen/analysis*
- Oxygen/pharmacology
- Ultraviolet Rays*
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/growth & development
- PubMed
- 27530196 Full text @ Environ. Toxicol. Chem.
Citation
Andrade, T.S., Henriques, J.F., Almeida, A.R., Soares, A.M., Scholz, S., Domingues, I. (2017) Zebrafish embryo tolerance to environmental stress factors - concentration/dose response analysis of oxygen limitation, pH and UV-light irradiation. Environmental toxicology and chemistry. 36(3):682-690.
Abstract
During the last century the increase in the mean global temperatures has been shown to impact on freshwater physico-chemical parameters such as pH, dissolved oxygen or UV light abundance. Changes in these parameters could modify the toxicity of environmental pollutants. Therefore, in this study, we studied the tolerance (survival and sublethal endpoints) of zebrafish (Danio rerio) embryos to variations in pH (3 - 12), dissolved oxygen (3.9-237 µmol/L) and UV intensity (55-467 mW/m(2) ) using selected endpoints. Sublethal endpoint assessment included the quantification of hatching success, developmental delay, reduction of body length, frequency of edema, and morphological abnormalities. 96 h-LC50 s of 3.68 and 10.21 were determined for acid and alkaline pH, respectively. Embryo survival appeared to be relatively resistant to oxygen depletion with a 96 h-LC50 of 0.42 mg/L. However, concentrations of 6 mg/L and below caused edema and developmental retardations. Continuous exposure to UV radiation (UVR) affected zebrafish development by reducing survival and hatching rate and triggering a series of developmental abnormalities such as pericardial edema and deformities. A 72 h-LC50 of 227 mW/m(2) was derived from intensity-response modelling. By generation of concentration-response parameters our data provide a basis for the subsequent assessment of combined effect of environmental stress parameters and chemicals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping