PUBLICATION
Globin mRNA reduction for whole-blood transcriptome sequencing
- Authors
- Krjut?kov, K., Koel, M., Roost, A.M., Katayama, S., Einarsdottir, E., Jouhilahti, E.M., Söderhäll, C., Jaakma, Ü., Plaas, M., Vesterlund, L., Lohi, H., Salumets, A., Kere, J.
- ID
- ZDB-PUB-160816-19
- Date
- 2016
- Source
- Scientific Reports 6: 31584 (Journal)
- Registered Authors
- Keywords
- Gene expression, RNA sequencing
- MeSH Terms
-
- RNA, Messenger/blood
- RNA, Messenger/chemistry*
- Transcriptome*
- High-Throughput Nucleotide Sequencing/methods*
- Female
- Humans
- Male
- Blood Cells/chemistry*
- Blood Cells/metabolism
- Globins/chemistry*
- PubMed
- 27515369 Full text @ Sci. Rep.
Citation
Krjut?kov, K., Koel, M., Roost, A.M., Katayama, S., Einarsdottir, E., Jouhilahti, E.M., Söderhäll, C., Jaakma, Ü., Plaas, M., Vesterlund, L., Lohi, H., Salumets, A., Kere, J. (2016) Globin mRNA reduction for whole-blood transcriptome sequencing. Scientific Reports. 6:31584.
Abstract
The transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robust and simple gmRNA reduction tool to preserve RNA quality, save time and cost. GL consists of a pair of gmRNA-specific oligonucleotides in RNA initial denaturation buffer that is effective immediately after RNA denaturation and adds only ten minutes of incubation to the whole cDNA synthesis procedure when compared to non-blood RNA analysis. We show that GL is fully effective not only for human samples but also for mouse and rat, and so far incompletely studied cow, dog and zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping