PUBLICATION
Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo
- Authors
- Sheng, F., Chen, M., Tan, Y., Xiang, C., Zhang, M., Li, B., Su, H., He, C., Wan, J., Li, P.
- ID
- ZDB-PUB-160810-5
- Date
- 2016
- Source
- Frontiers in pharmacology 7: 224 (Journal)
- Registered Authors
- Keywords
- Cynanchum otophyllum Schneid, apoptosis, epilepsy, neuroprotective effect, otophylloside N, pentylenetetrazol
- MeSH Terms
- none
- PubMed
- 27504096 Full text @ Front Pharmacol
Citation
Sheng, F., Chen, M., Tan, Y., Xiang, C., Zhang, M., Li, B., Su, H., He, C., Wan, J., Li, P. (2016) Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo. Frontiers in pharmacology. 7:224.
Abstract
Approximately 30% of epileptic patients worldwide are medically unable to control their seizures. In addition, repeated epileptic seizures generally lead to neural damage. Pentylenetetrazol (PTZ) is a clinical circulatory and respiratory stimulant that is experimentally used to mimic epileptic convulsion in epilepsy research. Here, we systematically explore the neuroprotective effects of a pure compound isolated from Cynanchum otophyllum Schneid (Qingyangshen), Otophylloside N (OtoN), against PTZ-induced neuronal injury. We used three models: in vitro primary cortical neurons, in vivo mice, and in vivo zebrafish. Our results revealed that OtoN treatment may attenuate PTZ-induced morphology changes, cell death, LDH efflux in embryonic neuronal cells of C57BL/6J mice, and convulsive behavior in zebrafish. Additionally, our Western blot and RT-PCR results demonstrated that OtoN may attenuate PTZ-induced apoptosis and neuronal activation in neuronal cells, mice, and zebrafish. OtoN may reduce PTZ-induced cleavage of poly ADP-ribose polymerase and upregulation of the Bax/Bcl-2 ratio and decrease the expression level of c-Fos. This study is the first investigation of the neuroprotective effects of OtoN, which might be developed as a novel antiepileptic drug.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping