ZFIN ID: ZDB-PUB-160731-4
Characterization of the POU5F1 homologue in Nile tilapia: from expression pattern to biological activity
Huang, X., Zhao, Y., Liu, L., Fan, Z., Zhou, L., Wang, Z., Wei, L., Wang, D., Wei, J.
Date: 2016
Source: Stem cells and development   25(18): 1386-95 (Journal)
Registered Authors:
Keywords: none
MeSH Terms:
  • Aging/metabolism
  • Amino Acid Sequence
  • Animals
  • Antibody Specificity/immunology
  • Base Sequence
  • Blastula/cytology
  • Cell Proliferation
  • Cell Survival
  • Cichlids/embryology
  • Cichlids/genetics*
  • DNA, Complementary/genetics
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental*
  • Germ Cells/metabolism
  • Gonads/metabolism
  • Octamer Transcription Factor-3/chemistry
  • Octamer Transcription Factor-3/genetics*
  • Octamer Transcription Factor-3/metabolism
  • Phylogeny
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid*
PubMed: 27473876 Full text @ Stem Cells Dev.
ABSTRACT
POU5F1 (OCT4) is a crucial transcription factor for induction and maintenance of cellular pluripotency as well as survival of germ cells in mammals. However, the homologues of POU5F1 in teleost fish including zebrafish and medaka, now named Pou5f3, exhibit considerable differences in expression pattern and pluripotency-maintaining activity. To what extent the POU5F1 homologues are conserved in vertebrates has been unclear. Here we report that the POU5F1 homologue from the Nile tilapia (Oreochromis niloticus), OnPou5f3,displays an expression pattern and biological activity somewhat different from those in zebrafish or medaka. The expression of Onpou5f3 at both mRNA and protein levels was abundant in early development embryos until blastula stages, barely detectable as proceeding, and then displayed a transiently strong expression domain in the brain region during neurula stages similar to zebrafish but not medaka. Afterwards, OnPou5f3 appeared germline-restricted (including PGCs, female and male gonad germ cells) expression just like medaka. Notably, OnPou5f3 depletion via morpholino oligos caused blastula blockage or lethality, and failure of survival and proliferation of blastula cell-derived cells. These findings indicate that equivalent POU5F1-like expression and activity of Pou5f3 might be conserved accompanying with species-specific expression pattern during evolution. Our study provides insight into the evolutionary conservation of the POU5F1 homologues across vertebrates.
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