|ZFIN ID: ZDB-PUB-160729-9|
Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish
Orger, M.B., Portugues, R.
|Source:||Methods in molecular biology (Clifton, N.J.) 1451: 307-20 (Chapter)|
|Registered Authors:||Orger, Mike|
|Keywords:||2-Photon microscopy, Behavior, Whole-brain imaging, Zebrafish|
|PubMed:||27464817 Full text @ Meth. Mol. Biol.|
Orger, M.B., Portugues, R. (2016) Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:307-20.
ABSTRACTWe present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.
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