PUBLICATION
Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish
- Authors
- Orger, M.B., Portugues, R.
- ID
- ZDB-PUB-160729-9
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 307-20 (Chapter)
- Registered Authors
- Orger, Mike
- Keywords
- 2-Photon microscopy, Behavior, Whole-brain imaging, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/physiology*
- Brain/metabolism
- Brain/physiology*
- Larva/physiology*
- Microscopy
- Zebrafish
- PubMed
- 27464817 Full text @ Meth. Mol. Biol.
Citation
Orger, M.B., Portugues, R. (2016) Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:307-20.
Abstract
We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping