ZFIN ID: ZDB-PUB-160729-9
Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish
Orger, M.B., Portugues, R.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 307-20 (Chapter)
Registered Authors: Orger, Mike
Keywords: 2-Photon microscopy, Behavior, Whole-brain imaging, Zebrafish
MeSH Terms:
  • Animals
  • Behavior, Animal/physiology*
  • Brain/metabolism
  • Brain/physiology*
  • Larva/physiology*
  • Microscopy
  • Zebrafish
PubMed: 27464817 Full text @ Meth. Mol. Biol.
ABSTRACT
We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.
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