Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels
- Horstick, E.J., Tabor, K.M., Jordan, D.C., Burgess, H.A.
- Methods in molecular biology (Clifton, N.J.) 1451: 355-66 (Chapter)
- Registered Authors
- Burgess, Harold, Horstick, Eric, Tabor, Kathryn
- Ablation, Isolation, Nav1.5, NfsB, Nitroreductase, SCN5a, Sensitization, Silencing, Tetrodotoxin, Zebrafish
- MeSH Terms
- Action Potentials/drug effects
- NAV1.5 Voltage-Gated Sodium Channel/metabolism
- Neurons/drug effects*
- Sodium Channel Blockers/pharmacology*
- 27464821 Full text @ Meth. Mol. Biol.
Horstick, E.J., Tabor, K.M., Jordan, D.C., Burgess, H.A. (2016) Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels. Methods in molecular biology (Clifton, N.J.). 1451:355-66.
Advances in genetic technologies enable the highly selective expression of transgenes in targeted neuronal cell types. Transgene expression can be used to noninvasively ablate, silence or activate neurons, providing a tool to probe their contribution to the control of behavior or physiology. Here, we describe the use of the tetrodotoxin (TTX)-resistant voltage-gated sodium channel Nav1.5 for either sensitizing neurons to depolarizing input, or isolating targeted neurons from surrounding neural activity, and methods for selective neuronal ablation using the bacterial nitroreductase NfsB.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes