ZFIN ID: ZDB-PUB-160729-5
Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels
Horstick, E.J., Tabor, K.M., Jordan, D.C., Burgess, H.A.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 355-66 (Chapter)
Registered Authors: Burgess, Harold, Horstick, Eric, Tabor, Kathryn
Keywords: Ablation, Isolation, Nav1.5, NfsB, Nitroreductase, SCN5a, Sensitization, Silencing, Tetrodotoxin, Zebrafish
MeSH Terms:
  • Action Potentials/drug effects
  • Animals
  • NAV1.5 Voltage-Gated Sodium Channel/metabolism
  • Neurons/drug effects*
  • Neurons/metabolism*
  • Nitroreductases/metabolism*
  • Sodium Channel Blockers/pharmacology*
  • Tetrodotoxin/pharmacology*
  • Zebrafish
PubMed: 27464821 Full text @ Meth. Mol. Biol.
ABSTRACT
Advances in genetic technologies enable the highly selective expression of transgenes in targeted neuronal cell types. Transgene expression can be used to noninvasively ablate, silence or activate neurons, providing a tool to probe their contribution to the control of behavior or physiology. Here, we describe the use of the tetrodotoxin (TTX)-resistant voltage-gated sodium channel Nav1.5 for either sensitizing neurons to depolarizing input, or isolating targeted neurons from surrounding neural activity, and methods for selective neuronal ablation using the bacterial nitroreductase NfsB.
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