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ZFIN ID: ZDB-PUB-160729-28
TALEN-Mediated Mutagenesis and Genome Editing
Ma, A.C., Chen, Y., Blackburn, P.R., Ekker, S.C.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 17-30 (Chapter)
Registered Authors: Blackburn, Patrick, Chen, Yi, Ekker, Stephen C.
Keywords: Customized nucleases, FusX, Genome editing, Golden Gate, TALEN, Zebrafish
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems/genetics
  • DNA Breaks, Double-Stranded
  • Gene Editing/methods*
  • Mutagenesis/genetics*
  • Transcription Activator-Like Effector Nucleases/genetics*
  • Zebrafish/genetics
PubMed: 27464798 Full text @ Meth. Mol. Biol.
Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifs for locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.