|ZFIN ID: ZDB-PUB-160729-28|
TALEN-Mediated Mutagenesis and Genome Editing
Ma, A.C., Chen, Y., Blackburn, P.R., Ekker, S.C.
|Source:||Methods in molecular biology (Clifton, N.J.) 1451: 17-30 (Chapter)|
|Registered Authors:||Blackburn, Patrick, Chen, Yi, Ekker, Stephen C.|
|Keywords:||Customized nucleases, FusX, Genome editing, Golden Gate, TALEN, Zebrafish|
|PubMed:||27464798 Full text @ Meth. Mol. Biol.|
Ma, A.C., Chen, Y., Blackburn, P.R., Ekker, S.C. (2016) TALEN-Mediated Mutagenesis and Genome Editing. Methods in molecular biology (Clifton, N.J.). 1451:17-30.
ABSTRACTTranscription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifs for locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.
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