PUBLICATION
Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae
- Authors
- Matty, M.A., Oehlers, S.H., Tobin, D.M.
- ID
- ZDB-PUB-160729-16
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 207-23 (Chapter)
- Registered Authors
- Matty, Molly, Oehlers, Stefan, Tobin, David
- Keywords
- Drug treatment, Infection, Innate immunity, Mycobacteria, Time-lapse imaging, Zebrafish
- MeSH Terms
-
- Immunity, Innate/genetics
- Immunity, Innate/physiology
- Zebrafish/immunology*
- Zebrafish/metabolism*
- Zebrafish/microbiology
- Larva/immunology*
- Larva/metabolism*
- Larva/microbiology
- Host-Pathogen Interactions/genetics
- Host-Pathogen Interactions/immunology
- Host-Pathogen Interactions/physiology*
- Animals
- Mycobacterium marinum/immunology
- Mycobacterium marinum/pathogenicity
- PubMed
- 27464810 Full text @ Meth. Mol. Biol.
Citation
Matty, M.A., Oehlers, S.H., Tobin, D.M. (2016) Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae. Methods in molecular biology (Clifton, N.J.). 1451:207-23.
Abstract
Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping