|ZFIN ID: ZDB-PUB-160729-16|
Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae
Matty, M.A., Oehlers, S.H., Tobin, D.M.
|Source:||Methods in molecular biology (Clifton, N.J.) 1451: 207-23 (Chapter)|
|Registered Authors:||Matty, Molly, Oehlers, Stefan, Tobin, David|
|Keywords:||Drug treatment, Infection, Innate immunity, Mycobacteria, Time-lapse imaging, Zebrafish|
|PubMed:||27464810 Full text @ Meth. Mol. Biol.|
Matty, M.A., Oehlers, S.H., Tobin, D.M. (2016) Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae. Methods in molecular biology (Clifton, N.J.). 1451:207-23.
ABSTRACTZebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
ADDITIONAL INFORMATION No data available