PUBLICATION
In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain
- Authors
- Zhang, R.W., Du, J.L.
- ID
- ZDB-PUB-160729-11
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 281-91 (Chapter)
- Registered Authors
- Du, Jiu Lin
- Keywords
- Neuron, Perforated patch-clamp recording, Whole-cell patch-clamp recording, Zebrafish larvae
- MeSH Terms
-
- Animals
- Brain/cytology*
- Brain/physiology*
- Larva/cytology
- Larva/physiology
- Neurons/cytology
- Neurons/physiology
- Patch-Clamp Techniques/methods*
- Synapses/metabolism
- Synapses/physiology
- Zebrafish
- PubMed
- 27464815 Full text @ Meth. Mol. Biol.
Citation
Zhang, R.W., Du, J.L. (2016) In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain. Methods in molecular biology (Clifton, N.J.). 1451:281-91.
Abstract
Zebrafish (Danio rerio) is a newly emerged vertebrate animal model with a conserved gross architecture of the brain and a rich repertoire of behaviors. Due to the optical transparency and structural simplicity of its brain, larval zebrafish has become an ideal in vivo model for dissecting neural mechanisms of brain functions at a whole-brain scale based on a strategy that spans scales from synapses, neurons, and circuits to behaviors. Whole-cell patch-clamp recording is an indispensable approach for studying synaptic and circuit mechanisms of brain functions. Due to the small size of neurons in the zebrafish brain, it is challenging to get whole-cell recordings from these cells. Here, we describe a protocol for obtaining in vivo whole-cell patch-clamp recordings from neurons in larval zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping