PUBLICATION

First report of Fusarium oxysporum species complex infection in zebrafish culturing system

Authors
Kulatunga, D.C., Dananjaya, S.H., Park, B.K., Kim, C.H., Lee, J., De Zoysa, M.
ID
ZDB-PUB-160728-29
Date
2017
Source
Journal of fish diseases   40(4): 485-494 (Journal)
Registered Authors
Kim, Cheol-Hee
Keywords
Danio rerio, Fusarium oxysporum, fungal hyphae, infection, internal transcribed spacer
MeSH Terms
  • Animals
  • DNA, Intergenic/genetics
  • Fish Diseases/microbiology*
  • Fish Diseases/prevention & control
  • Fusariosis/microbiology
  • Fusariosis/prevention & control
  • Fusariosis/veterinary*
  • Fusarium/classification
  • Fusarium/genetics
  • Fusarium/isolation & purification
  • Fusarium/physiology*
  • Phylogeny
  • Sequence Analysis, DNA/veterinary
  • Zebrafish*
PubMed
27451953 Full text @ J. Fish Dis.
Abstract
Fusarium oxysporum species complex (FOSC) is a highly diverse fungus. Recently, F. oxysporum infection was identified from zebrafish (Danio rerio) culturing system in Korea. Initially, a rapid whitish smudge was appeared in the water with the fungal blooming on walls of fish tanks. Microscopic studies were conducted on fungal hyphae, colony pigmentation and chlamydospore formation and the presence of macro- and microspores confirmed that the isolated fungus as F. oxysporum. Furthermore, isolated F. oxysporum was confirmed by internal transcribed spacer sequencing which matched (100%) to nine F. oxysporum sequences available in GenBank. Experimental hypodermic injection of F. oxysporum into adult zebrafish showed the development of fungal mycelium and pathogenicity similar to signs observed. Histopathologic results revealed a presence of F. oxysporum hyphae in zebrafish muscle. Fusarium oxysporum growth was increased with sea salt in a concentration-dependent manner. Antifungal susceptibility results revealed that F. oxysporum is resistant to copper sulphate (up to 200 μg mL-1 ) and sensitive to nystatin (up to 40 μg mL-1 ). This is the first report of FOSC from zebrafish culture system, suggesting it appears as an emerging pathogen, thus posing a significant risk on zebrafish facilities in the world.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes