PUBLICATION

Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis

Authors
Rochard, L., Monica, S.D., Ling, I.T., Kong, Y., Roberson, S., Harland, R., Halpern, M., Liao, E.C.
ID
ZDB-PUB-160722-12
Date
2016
Source
Development (Cambridge, England)   143: 2541-7 (Journal)
Registered Authors
Halpern, Marnie E., Harland, Richard, Liao, Eric, Roberson, Sara
Keywords
Convergent-extension, Morphogenesis, Palate, Wnt, Zebrabow
MeSH Terms
  • Morphogenesis/genetics*
  • Palate/embryology*
  • Palate/metabolism*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Phenotype
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Chondrocytes/metabolism
  • Wnt Signaling Pathway/genetics*
  • Cell Proliferation
  • Cell Shape
  • Epistasis, Genetic
  • Mutation/genetics
(all 15)
PubMed
27287801 Full text @ Development
Abstract
The Wnt signaling pathway is crucial for tissue morphogenesis, participating in cellular behavior changes, notably during the process of convergent-extension. Interactions between Wnt-secreting and receiving cells during convergent-extension remain elusive. We investigated the role and genetic interactions of Wnt ligands and their trafficking factors Wls, Gpc4 and Frzb in the context of palate morphogenesis in zebrafish. We describe that the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4. Using wls, gpc4, frzb, wnt9a and wnt5b mutants, we genetically dissected the Wnt signals operating between secreting ectoderm and receiving chondrocytes. Our analysis delineates that non-canonical Wnt signaling is required for cell intercalation, and that wnt5b and wnt9a are required for palate extension in the anteroposterior and transverse axes, respectively.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a131TgTransgenic Insertion
    c186
      Point Mutation
      hi1688TgTransgenic Insertion
      hi2735bTgTransgenic Insertion
      ir937TgTransgenic Insertion
        zf393TgTransgenic Insertion
          zf595TgTransgenic Insertion
            zf755
              Small Deletion
              zf756
                Indel
                zf757
                  Small Deletion
                  1 - 10 of 13
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  frzbCRISPR1-frzbCRISPR
                  wlsCRISPR1-wlsCRISPR
                  wnt9aCRISPR2-wnt9aCRISPR
                  wnt9aCRISPR3-wnt9aCRISPR
                  1 - 4 of 4
                  Show
                  Fish
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab1-wnt9apolyclonal
                    IgGRabbit
                    Ab3-wlspolyclonal
                      IgGRabbit
                      1 - 2 of 2
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CFPEFGCFP
                      CreEFGCre
                      EGFPEFGEGFP
                      KaedeEFGKaede
                      mCherryEFGmCherry
                      RFPEFGRFP
                      YFPEFGYFP
                      1 - 7 of 7
                      Show
                      Mapping
                      No data available