PUBLICATION
Spred-2 expression is associated with neural repair of injured adult zebrafish brain
- Authors
- Lim, F.T., Ogawa, S., Parhar, I.S.
- ID
- ZDB-PUB-160720-5
- Date
- 2016
- Source
- Journal of chemical neuroanatomy 77: 176-186 (Journal)
- Registered Authors
- Ogawa, Satoshi
- Keywords
- BrdU, ERK1/2, GFAP, in situ hybridization, neuroregeneration
- MeSH Terms
-
- Animals
- Antimetabolites/pharmacology
- Brain Chemistry/genetics
- Brain Injuries/genetics
- Brain Injuries/physiopathology
- Bromodeoxyuridine/pharmacology
- Cell Proliferation
- Gene Expression/genetics
- Glial Fibrillary Acidic Protein/metabolism
- MAP Kinase Signaling System/genetics
- Male
- Nerve Regeneration/genetics*
- Nerve Regeneration/physiology*
- Neurons/physiology*
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Repressor Proteins/biosynthesis*
- Repressor Proteins/genetics
- Repressor Proteins/physiology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 27427471 Full text @ J. Chem. Neuroanat.
Citation
Lim, F.T., Ogawa, S., Parhar, I.S. (2016) Spred-2 expression is associated with neural repair of injured adult zebrafish brain. Journal of chemical neuroanatomy. 77:176-186.
Abstract
Sprouty-related protein-2 (Spred-2) is a negative regulator of extracellular signal-regulated kinases (ERK) pathway, which is important for cell proliferation, neuronal differentiation, plasticity and survival. Nevertheless, its general molecular characteristics such as gene expression patterns and potential role in neural repair in the brain remain unknown. Thus, this study aimed to characterize the expression of spred-2 in the zebrafish brain. Digoxigenin-in situ hybridization showed spred-2 mRNA-expressing cells were mainly seen in the proliferative zones such as the olfactory bulb, telencephalon, optic tectum, cerebellum, and the dorsal and ventral hypothalamus, and most of which were neuronal cells. To evaluate the potential role of spred-2 in neuro-regeneration, spred-2 gene expression was examined in the dorsal telencephalon followed by mechanical-lesion. Real-time PCR showed a significant reduction of spred-2 mRNA levels in the telencephalon on 1-day till 2-days post-lesion and gradually increased to normal levels as compared with intact. Furthermore, to confirm involvement of Spred-2 signaling in the cell proliferation after brain injury, double-labeling of spred-2 in-situ hybridization with immunofluorescence of BrdU and phosphorylated-ERK1/2 (p-ERK1/2), a downstream of Spred-2 was performed. Increase of BrdU and p-ERK1/2 immunoreactive cells suggest that a decrease in spred-2 after injury might associated with activation of the ERK pathway to stimulate cell proliferation in the adult zebrafish brain. The present study demonstrates the possible role of Spred-2 signaling in cell proliferative phase during the neural repair in the injured zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping