PUBLICATION
Fluorescence-Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish
- Authors
- Bates, T.J., Naumann, U., Englert, C.
- ID
- ZDB-PUB-160716-8
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1467: 133-6 (Chapter)
- Registered Authors
- Englert, Christoph
- Keywords
- FACS, Podocytes, RNA isolation, Wt1a, qPCR
- MeSH Terms
-
- Animals
- Cell Separation/methods*
- Flow Cytometry/methods*
- Podocytes/cytology*
- Podocytes/metabolism
- WT1 Proteins/metabolism
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 27417966 Full text @ Meth. Mol. Biol.
Citation
Bates, T.J., Naumann, U., Englert, C. (2016) Fluorescence-Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish. Methods in molecular biology (Clifton, N.J.). 1467:133-6.
Abstract
Zebrafish is becoming a very important model for studying human diseases. The conserved structure of the nephrons in the kidney allows the user to answer questions relating to study human kidney disorders. Wt1a-expressing podocytes are the most important cells within the glomeruli of adult zebrafish. In order to understand the molecular characteristics of these cells, within damage models, we have established a method for isolating them.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping