PUBLICATION

CAT7 and cat7l long non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development

Authors
Ray, M.K., Wiskow, O., King, M.J., Ismail, N., Ergun, A., Wang, Y., Plys, A.J., Davis, C.P., Kathrein, K., Sadreyev, R., Borowsky, M.L., Eggan, K., Zon, L., Galloway, J.L., Kingston, R.E.
ID
ZDB-PUB-160715-8
Date
2016
Source
The Journal of biological chemistry   291(37): 19558-72 (Journal)
Registered Authors
Galloway, Jenna, Zon, Leonard I.
Keywords
CsCl density gradient, PRC1, chromatin, chromatin regulation, development, gene expression, long noncoding RNA (long ncRNA, lncRNA), mammal, polycomb, zebrafish
MeSH Terms
  • Humans
  • Neurons/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Gene Expression Regulation, Developmental/physiology*
  • Cell Differentiation/physiology*
  • Mice
  • Models, Biological*
  • RNA, Long Noncoding/genetics
  • RNA, Long Noncoding/metabolism*
  • HeLa Cells
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Polycomb Repressive Complex 1/genetics
  • Polycomb Repressive Complex 1/metabolism*
  • Animals
(all 16)
PubMed
27405765 Full text @ J. Biol. Chem.
Abstract
The essential functions of Polycomb Repressive Complex 1 (PRC1) in development and gene silencing are thought to involve long non-coding RNAs (lncRNAs), but few specific lncRNAs that guide PRC1 activity are known. We screened for lncRNAs which co-precipitate with PRC1 from chromatin and found candidates that impact Polycomb Group protein (PcG)-regulated gene expression in vivo. A novel lncRNA from this screen, CAT7, regulates expression and PcG binding at the MNX1 locus during early neuronal differentiation. CAT7 contains a unique tandem repeat domain which shares high sequence similarity to a non-syntenic zebrafish analog, cat7l. Defects caused by interference of cat7l RNA during zebrafish embryogenesis were rescued by human CAT7 RNA, enhanced by interference of a PRC1 component, and suppressed by interference of a known PRC1 target gene, demonstrating cat7l genetically interacts with a PRC1. We propose a model whereby PRC1 acts in concert with specific lncRNAs, and that CAT7/cat7l represent convergent lncRNAs that independently evolved to tune PRC1 repression at individual loci.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
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Mapping
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Citation
Ray, M.K., Wiskow, O., King, M.J., Ismail, N., Ergun, A., Wang, Y., Plys, A.J., Davis, C.P., Kathrein, K., Sadreyev, R., Borowsky, M.L., Eggan, K., Zon, L., Galloway, J.L., Kingston, R.E. (2016) CAT7 and cat7l long non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development. The Journal of biological chemistry. 291(37):19558-72.