PUBLICATION
Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish
- Authors
- Hu, P., Liu, M., Liu, Y., Wang, J., Zhang, D., Niu, H., Jiang, S., Wang, J., Zhang, D., Han, B., Xu, Q., Chen, L.
- ID
- ZDB-PUB-160701-12
- Date
- 2016
- Source
- Scientific Reports 6: 28952 (Journal)
- Registered Authors
- Wang, Jian
- Keywords
- Apoptosis, Gene expression, Gene expression profiling, Transcriptomics
- Datasets
- GEO:GSE69965
- MeSH Terms
-
- Animals
- Apoptosis
- Cold Temperature*
- Gene Expression Profiling
- Gene Regulatory Networks*
- Metabolic Networks and Pathways/genetics
- Stress, Physiological*
- Tilapia/genetics
- Tilapia/physiology
- Zebrafish/genetics
- Zebrafish/physiology
- PubMed
- 27356472 Full text @ Sci. Rep.
Citation
Hu, P., Liu, M., Liu, Y., Wang, J., Zhang, D., Niu, H., Jiang, S., Wang, J., Zhang, D., Han, B., Xu, Q., Chen, L. (2016) Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish. Scientific Reports. 6:28952.
Abstract
Transcriptional plasticity is a major driver of phenotypic differences between species. The lower temperature limit (LTL), namely the lower end of survival temperature, is an important trait delimiting the geographical distribution of a species, however, the genetic mechanisms are poorly understood. We investigated the inter-species transcriptional diversification in cold responses between zebrafish Danio rerio and tilapia Oreochromis niloticus, which were reared at a common temperature (28 °C) but have distinct LTLs. We identified significant expressional divergence between the two species in the orthologous genes from gills when the temperature cooled to the LTL of tilapia (8 °C). Five KEGG pathways were found sequentially over-represented in the zebrafish/tilapia divergently expressed genes in the duration (12 hour) of 8 °C exposure, forming a signaling cascade from metabolic regulation to apoptosis via FoxO signaling. Consistently, we found differential progression of apoptosis in the gills of the two species in which zebrafish manifested a delayed and milder apoptotic phenotype than tilapia, corresponding with a lower LTL of zebrafish. We identified diverged expression in 25 apoptosis-related transcription factors between the two species which forms an interacting network with diverged factors involving the FoxO signaling and metabolic regulation. We propose a genetic network which regulates LTL in fishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping