PUBLICATION

The first characterization of multidrug and toxin extrusion (MATE/SLC47) proteins in zebrafish (Danio rerio)

Authors
Lončar, J., Popović, M., Krznar, P., Zaja, R., Smital, T.
ID
ZDB-PUB-160701-10
Date
2016
Source
Scientific Reports   6: 28937 (Journal)
Registered Authors
Smital, Tvrtko
Keywords
Animal physiology, Transport carrier
MeSH Terms
  • Animals
  • Cloning, Molecular
  • Female
  • Gene Expression
  • HEK293 Cells
  • Humans
  • Kinetics
  • Liver/metabolism
  • Male
  • Organic Cation Transport Proteins/classification
  • Organic Cation Transport Proteins/genetics
  • Organic Cation Transport Proteins/metabolism*
  • Phylogeny
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
27357367 Full text @ Sci. Rep.
Abstract
Multidrug and toxin extrusion (MATE) proteins are involved in the extrusion of endogenous compounds and xenobiotics across the plasma membrane. They are conserved from bacteria to mammals, with different numbers of genes within groups. Here, we present the first data on identification and functional characterization of Mate proteins in zebrafish (Danio rerio). Phylogenetic analysis revealed six Mates in teleost fish, annotated as Mate3-8, which form a distinct cluster separated from the tetrapod MATEs/Mates. Synteny analysis showed that zebrafish mate genes are orthologous to human MATEs. Gene expression analysis revealed that all the mate transcripts were constitutively and differentially expressed during embryonic development, followed by pronounced and tissue-specific expression in adults. Functional analyses were performed using transport activity assays with model substrates after heterologous overexpression of five zebrafish Mates in HEK293T cells. The results showed that zebrafish Mates interact with both physiological and xenobiotic substances but also substantially differ with respect to the interacting compounds and interaction strength in comparison to mammalian MATEs/Mates. Taken together, our data clearly indicate a potentially important role for zebrafish Mate transporters in zebrafish embryos and adults and provide a basis for detailed functional characterizations of single zebrafish Mate transporters.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping