PUBLICATION
Zebrafish Embryo Disinfection with Povidone-Iodine: Evaluating an Alternative to Chlorine Bleach
- Authors
- Chang, C.T., Amack, J.D., Whipps, C.M.
- ID
- ZDB-PUB-160630-9
- Date
- 2016
- Source
- Zebrafish 13 Suppl 1: S96-S101 (Journal)
- Registered Authors
- Amack, Jeffrey
- Keywords
- none
- MeSH Terms
-
- Animals
- Chlorine/pharmacology
- Disinfectants/pharmacology
- Disinfection/methods*
- Fish Diseases/microbiology
- Fish Diseases/prevention & control*
- Mycobacterium Infections, Nontuberculous/microbiology
- Mycobacterium Infections, Nontuberculous/prevention & control
- Mycobacterium Infections, Nontuberculous/veterinary*
- Mycobacterium chelonae/drug effects
- Mycobacterium chelonae/physiology
- Mycobacterium marinum/drug effects
- Mycobacterium marinum/physiology
- Povidone-Iodine/pharmacology
- Zebrafish*
- PubMed
- 27351620 Full text @ Zebrafish
Citation
Chang, C.T., Amack, J.D., Whipps, C.M. (2016) Zebrafish Embryo Disinfection with Povidone-Iodine: Evaluating an Alternative to Chlorine Bleach. Zebrafish. 13 Suppl 1:S96-S101.
Abstract
Mycobacteriosis is a common bacterial infection in laboratory zebrafish caused by several different species and strains of Mycobacterium, including both rapid and slow growers. One control measure used to prevent mycobacterial spread within and between facilities is surface disinfection of eggs. Recent studies have highlighted the effectiveness of povidone-iodine (PVPI) on preventing propagation of Mycobacterium spp. found in zebrafish colonies. We evaluated the effect of disinfection using 12.5-50 ppm PVPI (unbuffered and buffered) on zebrafish exposed at 6 or 24 h postfertilization (hpf) to determine if this treatment is suitable for use in research zebrafish. Our results show that 6 hpf embryos are less sensitive to treatment as fewer effects on mortality, developmental delay, and deformity were observed. We also found that buffered PVPI treatment results in a greater knockdown of Mycobacterium chelonae and Mycobacterium marinum, as well as results in decreased harmful effects on embryos. Treatments of shorter (2 min vs. 5 min) duration were also more effective at killing mycobacteria in addition to resulting in fewer effects on embryo health. In addition, we compared the efficacy of a rinsing regimen to rinsing and disinfecting. Based on the findings of this study, we recommend disinfecting embryos for 2 min with buffered PVPI at 12.5-25 ppm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping