PUBLICATION
Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system
- Authors
- Løvmo, S.D., Speth, M.T., Repnik, U., Koppang, E.O., Griffiths, G.W., Hildahl, J.P.
- ID
- ZDB-PUB-160628-11
- Date
- 2017
- Source
- Developmental and comparative immunology 67: 508-518 (Journal)
- Registered Authors
- Hildahl, Jon
- Keywords
- Antigen sampling, Intestine, Mucosal immunity, Mycobacterium marinum, Nanoparticles, Zebrafish
- MeSH Terms
-
- Animals
- Antigen Presentation
- Bacterial Translocation/immunology
- Biological Transport
- Cells, Cultured
- Drug Delivery Systems
- Enterocytes/immunology*
- Fish Diseases/immunology*
- Immunity, Mucosal
- Intestinal Mucosa/microbiology
- Intestinal Mucosa/physiology*
- Mycobacterium Infections, Nontuberculous/immunology*
- Mycobacterium marinum/physiology*
- Nanoparticles/metabolism*
- Transendothelial and Transepithelial Migration
- Zebrafish/immunology*
- PubMed
- 27343826 Full text @ Dev. Comp. Immunol.
Citation
Løvmo, S.D., Speth, M.T., Repnik, U., Koppang, E.O., Griffiths, G.W., Hildahl, J.P. (2017) Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system. Developmental and comparative immunology. 67:508-518.
Abstract
Nano- and microparticles are promising carrier systems for oral delivery of drugs or vaccines, particularly in fish aquaculture. However, the mechanisms of uptake, trans-epithelial transport and immune response to nano/micrometer sized particles, or microorganisms such as bacteria are poorly understood in fish. Here, adult zebrafish were used to study the uptake of different nano- and microparticles and the pathogenic bacteria Mycobacterium marinum in the intestine, and their interactions with epithelial cells and the mucosal immune system. Fluorescent particles or bacteria were delivered directly into the adult zebrafish intestine by oral intubation and their localization was imaged in intestine, liver and spleen sections. Zebrafish do not appear to have M-cells, but both nanoparticles and bacteria were rapidly taken up in the intestine and transported to the liver and spleen. In each tissue, both bacteria and particles largely localized to leukocytes, presumably macrophages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping