PUBLICATION
Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure
- Authors
- Wu, S.M., Shu, L.H., Liu, J.H.
- ID
- ZDB-PUB-160623-8
- Date
- 2016
- Source
- Fish physiology and biochemistry 42(6): 1709-1720 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Cadmium, Metallothionein, Reactive oxygen species, Zebrafish
- MeSH Terms
-
- Glutathione Peroxidase/genetics
- Animals
- Reactive Oxygen Species/metabolism
- Protein Isoforms/genetics
- RNA, Messenger/metabolism
- Superoxide Dismutase/genetics
- Male
- Brain/drug effects*
- Brain/metabolism
- Apoptosis/drug effects
- Water Pollutants, Chemical/toxicity
- Gills/drug effects*
- Gills/metabolism
- Zebrafish/genetics*
- Zebrafish/metabolism
- Metallothionein/genetics*
- Gene Expression
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Catalase/genetics
- Cadmium/toxicity*
- PubMed
- 27329524 Full text @ Fish Physiol. Biochem.
Citation
Wu, S.M., Shu, L.H., Liu, J.H. (2016) Anti-oxidative functions of mt2 and smtB mRNA expression in the gills and brain of zebrafish (Danio rerio) upon cadmium exposure. Fish physiology and biochemistry. 42(6):1709-1720.
Abstract
There were not any past studies about metallothionein isoforms (smtB and mt2) having anti-oxidative functions on zebrafish after Cd2+ exposure. On the other hand, the anti-oxidative enzymatic factors such as superoxide dismutase (sod), glutathione peroxidase (gpx1a), and catalase (cat) are used as references to investigate whether the smtB and mt2 have anti-oxidative responses on the gills and brain of zebrafish after 1-6 h of 0 and 1.78 μM Cd2+ exposure. The anti-oxidative system such as sod, cat, and gpx1a mRNA expressions demonstrated a cascade response upon Cd2+-induced oxidative stress in the present study. Interestingly, the smtB mRNA expression levels increased by 3.2- to 6.1-fold, and mt2 raised by 4.1- to 11.3-fold in gills at 1 and 3 h after exposure to Cd2+, respectively. On the other hand, the smtB mRNA levels increased by 10.6- to 58.6-fold, but mt2 mRNA levels increased by 2.3- to 11.1-fold in brain at 1 and 3 h after exposure to Cd2+, respectively. In addition, both tissues showed increased apoptosis levels at 3 h, and recovery after 6 h of Cd2+ exposure. From the results, we suggest that both mt2 and smtB play a role in anti-oxidation responses within 6 h after exposure to Cd2+. In conclusion, the smtB mRNA levels have a higher response than mt2 in the brain, but both mRNA expressions appear to have a similar pattern in the gill. We suggest that smtB plays an important role to defend oxidative stress in the brain of adult zebrafish upon acute Cd2+ exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping