PUBLICATION
Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development
- Authors
- Macaulay, L.J., Chernick, M., Chen, A., Hinton, D.E., Bailey, J.M., Kullman, S.W., Levin, E.D., Stapleton, H.M.
- ID
- ZDB-PUB-160623-11
- Date
- 2017
- Source
- Environmental toxicology and chemistry 36(1): 36-48 (Journal)
- Registered Authors
- Keywords
- PBDE, development, juvenile, mixture, zebrafish
- MeSH Terms
-
- Adult
- Animals
- Dose-Response Relationship, Drug
- Gene Expression/drug effects
- Halogenated Diphenyl Ethers/metabolism
- Halogenated Diphenyl Ethers/toxicity*
- Humans
- Osteogenesis/drug effects*
- Osteogenesis/genetics
- Polybrominated Biphenyls/metabolism
- Polybrominated Biphenyls/toxicity*
- Thyroid Gland/drug effects
- Thyroid Gland/metabolism
- Thyroid Hormones/genetics
- Thyroid Hormones/metabolism*
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- PubMed
- 27329031 Full text @ Environ. Toxicol. Chem.
Citation
Macaulay, L.J., Chernick, M., Chen, A., Hinton, D.E., Bailey, J.M., Kullman, S.W., Levin, E.D., Stapleton, H.M. (2017) Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish (Danio rerio) development. Environmental toxicology and chemistry. 36(1):36-48.
Abstract
Polybrominated diphenyl ethers (PBDEs) and their metabolites (e.g. hydroxylated BDEs (OH-BDEs)) are contaminants detected together frequently in human tissues, and are structurally similar to thyroid hormones (TH). THs partially mediate metamorphic transitions between life stages in zebrafish, making this a critical developmental window which may be vulnerable to chemicals disrupting thyroid signaling. In this study, zebrafish were exposed to 6-OH-BDE-47 (30 nM) alone or to a low (30 µg/L) or high dose (600 µg/L) mixture of PentaBDEs, 6-OH-BDE-47 (0.5-6 µg/L), & 2,4,6 tribromophenol (TBP) (5-100 µg/L) during juvenile development (9-23 dpf) and evaluated for developmental endpoints mediated by TH signaling. Fish were sampled at three time points and examined for developmental and skeletal morphology, apical thyroid and skeletal gene markers, and modifications in swimming behavior (as adults). Exposure to the high mixture resulted in > 85% mortality within one week of exposure, despite being below reported acute toxicity thresholds for individual congeners. The low mixture and 6-OH-BDE-47 groups exhibited reductions in body length and delayed maturation, specifically relating to swim bladder, fin, and pigmentation development. Reduced skeletal ossification was also observed in 6-OH-BDE-47 treated fish. Assessment of thyroid and osteochondral gene regulatory networks demonstrated significantly increased expression of genes that regulate skeletal development and THs. Overall, these results indicate that exposures to PBDEs/OH-BDEs mixtures adversely impact zebrafish maturation during metamorphosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping