ZFIN ID: ZDB-PUB-160613-1
Fatty liver disease induced by perfluorooctane sulfonate: Novel insight from transcriptome analysis
Fai Tse, W.K., Li, J.W., Kwan Tse, A.C., Chan, T.F., Hin Ho, J.C., Sun Wu, R.S., Chu Wong, C.K., Lai, K.P.
Date: 2016
Source: Chemosphere   159: 166-177 (Journal)
Registered Authors:
Keywords: Environmental pollutant, Hepatotoxicity, Perfluorooctane sulfonate, Transcriptome, Zebrafish
MeSH Terms:
  • Alkanesulfonic Acids/toxicity*
  • Animals
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology
  • Fatty Liver/chemically induced
  • Fatty Liver/genetics
  • Fatty Liver/pathology*
  • Fluorocarbons/toxicity*
  • Gene Expression Profiling*
  • Hepatitis/etiology
  • Hepatitis/pathology*
  • Hepatocytes
  • Humans
  • Liver/drug effects
  • Liver/metabolism*
  • Liver Cirrhosis/chemically induced
  • Liver Cirrhosis/genetics
  • Liver Cirrhosis/pathology*
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/metabolism
PubMed: 27289203 Full text @ Chemosphere
Perfluorooctane sulfonate (PFOS), a hepato-toxicant and potential non-genotoxic carcinogen, was widely used in industrial and commercial products. Recent studies have revealed the ubiquitous occurrence of PFOS in the environment and in humans worldwide. The widespread contamination of PFOS in human serum raised concerns about its long-term toxic effects and its potential risks to human health. Using fatty liver mutant foie gras (fgr(-/-))/transport protein particle complex 11 (trappc11(-/-)) and PFOS-exposed wild-type zebrafish embryos as the study model, together with RNA sequencing and comparative transcriptomic analysis, we identified 499 and 1414 differential expressed genes (DEGs) in PFOS-exposed wild-type and trappc11 mutant zebrafish, respectively. Also, the gene ontology analysis on common deregulated genes was found to be associated with different metabolic processes such as the carbohydrate metabolic process, glycerol ether metabolic process, mannose biosynthetic process, de novo' (Guanosine diphosphate) GDP-l-fucose biosynthetic process, GDP-mannose metabolic process and galactose metabolic process. Ingenuity Pathway Analysis further highlighted that these deregulated gene clusters are closely related to hepatitis, inflammation, fibrosis and cirrhosis of liver cells, suggesting that PFOS can cause liver pathogenesis and non-alcoholic fatty liver disease in zebrafish. The transcriptomic alterations revealed may serve as biomarkers for the hepatotoxic effect of PFOS.