PUBLICATION

Using fluorescent lipids in live zebrafish larvae: From imaging whole animal physiology to subcellular lipid trafficking

Authors
Anderson, J.L., Carten, J.D., Farber, S.A.
ID
ZDB-PUB-160608-13
Date
2016
Source
Methods in cell biology   133: 165-178 (Chapter)
Registered Authors
Anderson, Jennifer, Carten, Juliana, Farber, Steven
Keywords
BODIPY, Cholesterol, Fat, Fatty acid, Intestine, Lipid drop, Liver, Triacylglycerol, Zebrafish
MeSH Terms
  • Animals
  • Larva/metabolism
  • Lipid Metabolism
  • Lipids/analysis*
  • Optical Imaging/methods*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
PubMed
27263413 Full text @ Meth. Cell. Biol.
Abstract
Lipids serve essential functions in cells as signaling molecules, membrane components, and sources of energy. Defects in lipid metabolism are implicated in a number of pandemic human diseases, including diabetes, obesity, and hypercholesterolemia. Many aspects of how fatty acids and cholesterol are absorbed and processed by intestinal cells remain unclear and present a hurdle to developing approaches for disease prevention and treatment. Numerous studies have shown that the zebrafish is an excellent model for vertebrate lipid metabolism. In this chapter, we review commercially available fluorescent lipids that can be deployed in live zebrafish to better understand lipid signaling and metabolism. In this chapter, we present criteria one should consider when selecting specific fluorescent lipids for the study of digestive physiology or lipid metabolism in larval zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes