PUBLICATION

Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function

Authors
Chen, Y., Li, J., Li, Q., Wang, T., Xing, L., Xu, H., Wang, Y., Shi, Q., Zhou, Q., Liang, Q.
ID
ZDB-PUB-160531-6
Date
2016
Source
Evidence-based complementary and alternative medicine : eCAM   2016: 7067691 (Journal)
Registered Authors
Li, Qiang
Keywords
none
MeSH Terms
none
PubMed
27239212 Full text @ Evid. Based Complement. Alternat. Med.
Abstract
To investigate whether Du-Huo-Ji-Sheng-Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3-month-old TNF-Tg mice with DHJST (12 g/kg) or the same volume of physiological saline once every day for 12 weeks, and 3-month-old WT littermates were used as negative control. After twelve weeks, we performed NIR-ICG imaging and found that DHJST increased the ICG clearance at the footpad and the pulse of efferent lymphatic vessel between popliteal lymph node and footpad. Histology staining at ankle joints showed that DHJST decreases synovial inflammation, bone erosion, cartilage erosion, and TRAP+ osteoclast area in TNF-Tg mice. Immunohistochemical staining by using anti-Lyve-1 and anti-podoplanin antibody showed that DHJST stimulated lymphangiogenesis in ankle joints of TNF-Tg mice. And zebrafish study suggested that DHJST promoted the formation of lymphatic thoracic duct. In conclusion, DHJST inhibits inflammation severity and promotes lymphangiogenesis and lymphatic drainage function of TNF-Tg mice.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
sd2Tg; y1TgcontrolLong-pecIFL
sd2Tg; y1TgcontrolLong-pecIFL
1 - 2 of 2
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sd2TgTransgenic Insertion
    y1TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      Human Disease Fish Conditions Evidence
      rheumatoid arthritisTAS
      1 - 1 of 1
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      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      sd2Tg; y1Tg
      1 - 1 of 1
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      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      DsRedEFGDsRed
      EGFPEFGEGFP
      1 - 2 of 2
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      Mapping
      No data available
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      Citation
      Chen, Y., Li, J., Li, Q., Wang, T., Xing, L., Xu, H., Wang, Y., Shi, Q., Zhou, Q., Liang, Q. (2016) Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function. Evidence-based complementary and alternative medicine : eCAM. 2016:7067691.